Interleukin 27 attenuates collagen-induced arthritis
Interleukin 27 attenuates collagen-induced arthritis
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DOI：
10.1136/ard.2007.083360
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发表时间：
2008-10-01
影响因子：
27.4
通讯作者：
Liew, F. Y.
中科院分区：
文献类型：
--
作者：
Niedbala, W.;Cai, B.;Liew, F. Y.
目的：为探讨白细胞介素27（IL 27）在类风湿关节炎（RA）中的作用，采用胶原诱导的关节炎（CIA）小鼠模型，观察IL 27在RA患者关节中的表达及重组IL 27的体内作用。用IL 27处理发展CIA的小鼠，并确定随后的疾病进展和免疫学特征。结果：IL-27在RA滑膜中表达明显。与未治疗的对照组相比，在疾病发作时短期施用IL 27显著减轻了疾病的严重程度。组织学检查显示，虽然未处理的小鼠在关节中发生了严重的细胞浸润、滑膜增生和关节侵蚀，但这种病理在IL 27处理的动物中显著减少。用IL 27治疗小鼠还降低了血清IL 6和胶原特异性IgG 2a的量。IL-27处理的小鼠脾和淋巴结细胞产生的干扰素c和IL-17显着低于从对照小鼠的细胞时，与胶原蛋白在vitro cultured.Conclusion：这些结果表明，IL-27可能是一个潜在的治疗剂对RA在发病。
Objective: To investigate the potential role of interleukin (IL) 27 in rheumatoid arthritis (RA) by examining the expression of IL27 in the articular joints of patients with RA and the effect of recombinant IL27 in vivo in a murine model of collagen-induced arthritis (CIA).Methods: Synovial membranes from patients with RA were examined for the presence of IL27 by immunohistochemistry and by western blot. Mice developing CIA were treated with IL27 and the ensuing disease progression and immunological profile determined. The effect of IL27 on T-cell response in vitro was also ascertained.Results: IL27 was clearly detected in the RA synovial membranes. Short-term administration of IL27 at the onset of the disease significantly attenuated disease severity compared with untreated controls. Histological examination showed that while untreated mice developed severe cellular infiltration in the joints, synovial hyperplasia and joint erosion, this pathology was profoundly reduced in IL27-treated animals. Treatment of mice with IL27 also decreased the amounts of serum IL6 and collagen-specific IgG2a. Spleen and lymph node cells from the IL27-treated mice produced significantly less interferon c and IL17 than cells from the control mice when cultured with collagen in vitro.Conclusion: These results demonstrate that IL27 may be a potential therapeutic agent against RA at the onset of the disease.