Heterotransplantation of small‐cell carcinoma of the lung into nude mice: Comparison of intracranial and subcutaneous routes
Heterotransplantation of small‐cell carcinoma of the lung into nude mice: Comparison of intracranial and subcutaneous routes
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小细胞肺癌裸鼠异种移植：颅内和皮下途径的比较
DOI：
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发表时间：
1981
影响因子：
6.4
通讯作者：
Alfreda D. Simmons
中科院分区：
文献类型：
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作者：
A. Gazdar;D. Carney;Harvie L. Sims;Alfreda D. Simmons
我们比较和对比了小细胞肺癌（SCCL）颅内（i.c）和皮下（s.c）异源移植到胸腺裸鼠体内的效果。使用新鲜人SCCL肿瘤标本、软琼脂糖培养的肿瘤菌落和连续细胞系。三种标本诱导的肿瘤相似，但s.c.和i.c.移植不同。sc肿瘤具有较长的潜伏时间，非侵袭性和非致命性。L.c.肿瘤潜伏期较短，总是在脑膜中生长，经常侵入并破坏底层大脑，是致命的。icc移植诱导肿瘤的剂量比sc移植低10 ~ 1000倍。将在软琼脂糖中培养的SCCL肿瘤标本集合集落接种。虽然它们含有相对较少的细胞（400-10,000），但这些集落标本中有83%在58-243天后诱导肿瘤，证实了集落的“干细胞”来源。lc和sc移植保留了SCCL的特征形态，并且除了一个例外，没有转移到远处器官。两种类型的移植都可以很容易地建立连续的细胞系，并且它们保留了原始SCCL肿瘤的细胞学、生长和生化特性。SCCL的l.c.异源移植是一种有用的工具，特别是当肿瘤细胞数量较少时，可能为研究脑膜癌病的生物学和治疗提供模型。
We compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small‐cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types of specimens were similar, but s.c. and i.c. transplants differed. S.c. tumors had longer latent times, were non‐invasive and non‐lethal. l.c. tumors had shorter latent periods, invariably grew in the meninges, frequently invaded and destroyed the underlying brain, and were lethal. The tumor‐inducing dose for i.c. transplantation was 10 to 1,000 times lower than for s.c. transplantation. Pooled colonies of SCCL tumor specimens grown in soft agarose were inoculated i.c. While they contained relatively small numbers of cells (400–10,000), 83% of these colony specimens induced tumors after 58–243 days, confirming the «stem‐cell» origin of the colonies. l.c. and s.c. transplants retained the characteristic morphology of SCCL, and, with one exception, did not metastasize to distant organs. Continous cell lines could be established readily from both types of transplants, and they retained the characteristic cytology, growth and biochemical properties of the original SCCL tumors. l.c. heterotransplantation of SCCL is a useful tool, especially when small numbers of tumor cells are available, and may provide a model to study the biology and therapy of meningeal carcinomatosis.
来自不同人类实体瘤活检的软琼脂中细胞集落的生长。
影响因子：
11.2
作者：
Pavelic,ZP;Slocum,HK;Rustum,YM;Creaven,PJ;Nowak,NJ;Karakousis,C;Takita,H;Mittelman,A
通讯作者：
Mittelman,A