GINS, a central nexus in the archaeal DNA replication fork
GINS, a central nexus in the archaeal DNA replication fork
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DOI：
10.1038/sj.embor.7400649
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发表时间：
2006-05-01
期刊：
影响因子：
7.7
通讯作者：
Bell, SD
中科院分区：
文献类型：
--
作者：
Marinsek, N;Barry, ER;Bell, SD
在真核生物中，GINS 复合物对于 DNA 复制至关重要，并且被认为在复制叉中发挥作用。该复合物由四个旁系同源 GINS 亚基 Psf1、Psf2、Psf3 和 SId5 组成。在这里，我们确定了古菌 GINS 同源物作为 MCM 解旋酶的直接相互作用伙伴。核心古菌 GINS 复合物包含两个亚基，它们是真核 GINS 亚基的保守性较差的同源物，与含有与细菌 RecJ 的 DNA 结合结构域同源的结构域的蛋白质形成复合物。相互作用研究表明古菌 GINS 直接与异二聚体核心引物酶相互作用。我们的数据表明，GINS 对于协调复制叉的结构很重要，并提供了一种机制，将前导链上的 MCM 解旋酶的进展与滞后链上的引发事件耦合起来。
In eukaryotes, the GINS complex is essential for DNA replication and has been implicated as having a role at the replication fork. This complex consists of four paralogous GINS subunits, Psf1, Psf2, Psf3 and SId5. Here, we identify an archaeal GINS homologue as a direct interaction partner of the MCM helicase. The core archaeal GINS complex contains two subunits that are poorly conserved homologues of the eukaryotic GINS subunits, in complex with a protein containing a domain homologous to the DNA-binding domain of bacterial RecJ. Interaction studies show that archaeal GINS interacts directly with the heterodimeric core primase. Our data suggest that GINS is important in coordinating the architecture of the replication fork and provide a mechanism to couple progression of the MCM helicase on the leading strand with priming events on the lagging strand.