Protein crystallography beamline BL2S1 at the Aichi synchrotron.
Protein crystallography beamline BL2S1 at the Aichi synchrotron.
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DOI：
10.1107/s1600577516018579
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发表时间：
2017-01-01
影响因子：
2.5
通讯作者：
Tabuchi M
中科院分区：
文献类型：
--
作者：
Watanabe N;Nagae T;Yamada Y;Tomita A;Matsugaki N;Tabuchi M
在AichiSR的5 T超弯口上构建了蛋白质晶体学光束线BL2S1。蛋白质晶体学光束线BL2S1是在爱知同步加速器的5t超导弯曲磁体端口之一上构建的，可供学术和工业组织的用户使用。光束线主要用于大分子（如蛋白质和核酸）单晶的x射线衍射测量。其他材料晶体的衍射测量也是可能的，例如无机和有机化合物。BL2S1采用非对称切割弯曲单晶单色仪[Ge（111）或Ge(220)]，覆盖7-17 keV （1.8-0.7 Å）的能量范围，镀铂硅镜用于垂直聚焦和作为高阶截止滤波器。光束线配备了一个单轴测角仪，一个CCD探测器和一个开放流低温样品冷却器。高压蛋白质晶体学与金刚石砧细胞也可以执行使用该光束线。
The protein crystallography beamline BL2S1 has been constructed on the 5 T superbend port of the AichiSR. The protein crystallography beamline BL2S1, constructed at one of the 5 T superconducting bending-magnet ports of the Aichi synchrotron, is available to users associated with academic and industrial organizations. The beamline is mainly intended for use in X-ray diffraction measurements of single-crystals of macromolecules such as proteins and nucleic acids. Diffraction measurements for crystals of other materials are also possible, such as inorganic and organic compounds. BL2S1 covers the energy range 7–17 keV (1.8–0.7 Å) with an asymmetric-cut curved single-crystal monochromator [Ge(111) or Ge(220)], and a platinum-coated Si mirror is used for vertical focusing and as a higher-order cutoff filter. The beamline is equipped with a single-axis goniometer, a CCD detector, and an open-flow cryogenic sample cooler. High-pressure protein crystallography with a diamond anvil cell can also be performed using this beamline.