Acetylcholine-dependent induction and expression of functional plasticity in the barrel cortex of the adult rat
Acetylcholine-dependent induction and expression of functional plasticity in the barrel cortex of the adult rat
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DOI：
10.1152/jn.2001.86.1.422
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发表时间：
2001-07-01
影响因子：
2.5
通讯作者：
Ahissar, E
中科院分区：
文献类型：
--
作者：
Ego-Stengel, V;Shulz, DE;Ahissar, E
乙酰胆碱（ACh）参与神经元感觉可塑性的诱导是有据可查的。最近，我们证明在麻醉大鼠的躯体感觉皮层中，ACh也参与神经元可塑性的表达。配对刺激的主须在一个固定的时间频率与乙酰胆碱离子电渗诱导增强反应，需要重新应用乙酰胆碱表达。在这里，我们充分表征这种现象，并将其扩展到刺激相邻的晶须。我们表明，这些乙酰胆碱依赖的增强是累积的和可逆的。当连续施加几个感觉胆碱能配对与刺激的主须，在配对频率的响应进一步增加，表现出一个累积的过程，可以达到饱和水平。增强作用是特定于刺激频率的：如果连续的配对是在不同的频率下进行的，那么由第一对引起的增强作用是去增强的，而对新配对频率的反应是增强的。在测试过程中，增强的反应并没有立即开发的配对频率的演示文稿在应用乙酰胆碱：动力学的效果的分析表明，这个过程需要顺序演示的几个列车的刺激在成对的频率来表达。我们提出的证据表明，具有类似特征的可塑性，可以诱导的反应，刺激相邻的胡须，这表明，这种增强可能参与感受野空间重组。这里提出的乙酰胆碱依赖的可塑性的空间和时间特性施加特定的约束基础的细胞和分子机制。
The involvement of acetylcholine (ACh) in the induction of neuronal sensory plasticity is well documented. Recently we demonstrated in the somatosensory cortex of the anesthetized rat that ACh is also involved in the expression of neuronal plasticity. Pairing stimulation of the principal whisker at a fixed temporal frequency with ACh iontophoresis induced potentiations of response that required re-application of ACh to be expressed. Here we fully characterize this phenomenon and extend it to stimulation of adjacent whiskers. We show that these ACh-dependent potentiations are cumulative and reversible. When several sensori-cholinergic pairings were applied consecutively with stimulation of the principal whisker, the response at the paired frequency was further increased, demonstrating a cumulative process that could reach saturation levels. The potentiations were specific to the stimulus frequency: if the successive pairings were done at different frequencies, then the potentiation caused by the first pairing was depotentiated, whereas the response to the newly paired frequency was potentiated. During testing, the potentiation of response did not develop immediately on the presentation of the paired frequency during application of ACh: the analysis of the kinetics of the effect indicates that this process requires the sequential presentation of several trains of stimulation at the paired frequency to be expressed. We present evidence that a plasticity with similar characteristics can be induced for responses to stimulation of an adjacent whisker, suggesting that this potentiation could participate in receptive field spatial reorganizations. The spatial and temporal properties of the ACh-dependent plasticity presented here impose specific constraints on the underlying cellular and molecular mechanisms.