Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
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DOI：
10.1016/j.jviromet.2010.01.025
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发表时间：
2010-07-01
影响因子：
3.1
通讯作者：
Yoshikawa, Tetsushi
中科院分区：
文献类型：
--
作者：
Ihira, Masaru;Sugiyama, Hiroko;Yoshikawa, Tetsushi
建立了变异特异性直接环介导等温扩增（LAMP）方法，用于检测血清样本中人类疱疹病毒6型（HHV-6）变异体。针对HHV-6 U86基因设计特异性引物。初步验证分析证实了该方法的高特异性和灵敏度。该方法用于监测造血干细胞移植受者活动性HHV-6感染具有较高的可靠性。(C)2010 Elsevier B. V.保留所有权利。
A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients. (C) 2010 Elsevier B.V. All rights reserved.