Morphological and molecular characterisation of Hemicriconemoides paracamelliae sp n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides from China
Morphological and molecular characterisation of Hemicriconemoides paracamelliae sp n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides from China
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DOI：
10.1163/15685411-00003147
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发表时间：
2018-01-01
期刊：
影响因子：
1.2
通讯作者：
Zheng, Jingwu
中科院分区：
文献类型：
--
作者：
Maria, Munawar;Cai, Ruihang;Zheng, Jingwu
半角线虫属的鞘状线虫是许多作物的迁徙根外寄生虫，但仅对少数物种造成损害。副茶半角蝽属 sp. n.，分离于中国浙江杭州，从 Cinninghamia lanceolata 的根际中分离出来，与 H. kanayaensis 和 H. parataiwanensis 一起被描述。这三个物种具有形态学和分子学特征，并通过 SEM 照片阐明了重要的形态学细节。该新物种的特征可以是正面视图，其具有带狭缝状开口的背腹定向口盘，并且唇板由口盘侧面上的两个侧向半球形突起组成。该新种第一唇环扩大，略宽于第二唇环，管心针长83（80-85）μm，排泄孔位于咽球基部后5-6环，外阴狭缝状，无外阴瓣，受精囊圆形至长圆形，肛门位于外阴后5-8环。尾部细长，圆锥形，末端环向背侧或腹侧弯曲，形成细圆的尖端。该研究提供了来自中国大陆的H. kanayaensis的首次记录，这是H. parataiwanensis的一个新宿主群落，以及18S、28S D2-D3和ITS序列的分子测序数据。
Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many crops but damage is documented for only a few species. Hemicriconemoides paracamelliae sp. n., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of Cinninghamia lanceolata, is described together with H. kanayaensis and H. parataiwanensis. These three species are characterised morphologically and molecularly with important morphological details elucidated by SEM photographs. The new species can be characterised by the en face view having a dorsoventrally orientated oral disc with slit-like opening, and the labial plate being composed of two lateral semi-globular shaped projections on the lateral sides of the oral disc. This new species has the first lip annulus expanded and slightly wider than the second, stylet 83 (80-85) mu m long, excretory pore located 5-6 annuli posterior to the base of the pharyngeal bulb, vulva slit-like, vulval flaps absent, spermatheca rounded to oblong-shaped, and anus located 5-8 annuli posterior to vulva. The tail is elongated, conoid, with the terminal annuli curving dorsally or ventrally to form a finely rounded tip. The study provides the first record of H. kanayaensis from mainland China, a new host association of H. parataiwanensis, and molecular sequencing data of the 18S, 28S D2-D3 and ITS sequences.