Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis
Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis
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DOI：
10.1016/j.abb.2020.108434
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发表时间：
2020-08-15
影响因子：
3.9
通讯作者：
Cang, Shundong
中科院分区：
文献类型：
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作者：
Lu, Chuangxin;Fu, Lei;Cang, Shundong
背景：环状RNA（circRNA）被认为参与了肿瘤的发生。本研究旨在探讨circ-FARSA在结直肠癌中的功能意义和调控作用。方法：采用定量逆转录聚合酶链反应（RT-PCR）和Western blot检测circ-FARSA基因表达。结果：circ-FARSA在结直肠癌组织和细胞系中表达上调，其表达与结直肠癌患者的总生存期密切相关。敲低circ-FARSA抑制了体外CRC细胞的增殖、迁移和侵袭。此外，circ-FARSA作为miR-330- 5 p的海绵，其上调减轻了miR-330- 5 p对CRC细胞增殖和转移的抑制作用。此外，circ-FARSA通过海绵状的miR-330- 5 p调节LIM和SH 3蛋白1（LASP 1）的表达。结论：circ-FARSA基因沉默可通过调控miR-330- 5 p/LASP 1轴抑制结直肠癌的生长，为结直肠癌的发生提供了一种新的调控机制。
Background: Circular RNA (circRNA) has been proposed to be involved in carcinogenesis. Here, we explored the functional significance and regulatory role of circ-FARSA in colorectal cancer (CRC).Methods: Gene expression was determined using quantitative reverse transcriptase polymerase chain reaction and Western blot. We determined the effect of circFARSA on CRC progression using cell count kit-8, colony formation assay, wound-healing assay, transwell invasion assay, luciferase reporter assay and in vivo assay.Result: circ-FARSA was upregulated in CRC tissues and cell lines, and its expression had a significant association with the overall survival of CRC patients. Knockdown of circ-FARSA inhibited the proliferation, migration, and invasion of CRC cells in vitro. Moreover, circ-FARSA functioned as a sponge of miR-330-5p, and its upregulation mitigated the inhibitory effects of miR-330-5p on CRC cell proliferation and metastasis. In addition, circ-FARSA regulated the expression of LIM and SH3 protein 1 (LASP1) by sponging miR-330-5p. Besides, inhibition of circFARSA repressed the growth of CRC in vivo.Conclusion: Silencing of circ-FARSA restricted the growth of CRC through regulating the miR-330-5p/LASP1 axis, providing a novel regulatory mechanism for CRC tumorigenesis.