Naringin Protects Against High Glucose-Induced Human Endothelial Cell Injury Via Antioxidation and CX3CL1 Downregulation
Naringin Protects Against High Glucose-Induced Human Endothelial Cell Injury Via Antioxidation and CX3CL1 Downregulation
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柚皮苷通过抗氧化和 CX3CL1 下调来防止高血糖引起的人内皮细胞损伤
DOI：
10.1159/000480215
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发表时间：
2017-01-01
影响因子：
--
通讯作者：
Zhu, Gaochun
中科院分区：
文献类型：
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作者：
Li, Guilin;Xu, Yurong;Zhu, Gaochun
背景/目的：糖尿病高血糖诱导内皮损伤已被广泛接受。柚皮苷是一种生物类黄酮。一些研究表明，柚苷减轻糖尿病并发症，但柚苷改善糖尿病异常的确切机制尚未完全了解。本研究旨在研究柚皮苷对高糖诱导的人脐静脉内皮细胞（HUVECs）损伤的保护作用。方法：HUVECs在高糖或高糖条件下培养5天，同时加入或不加入柚皮苷。通过实时定量RT-PCR（qPCR）和western blot检测CX 3CL 1的表达。用Seahorse Bioscience XF分析仪测量细胞生物能量分析耗氧率（OCR）。结果如下：高糖培养的HUVECs活性氧（ROS）的产生、CX 3CL 1的表达及AKT磷酸化水平均较对照组增加。然而，柚苷挽救了ROS产生，CX 3CL 1表达和AKT磷酸化的增加。高糖组一氧化氮（NO）生成和OCR降低，柚皮苷可恢复高糖引起的变化。分子对接结果表明，柚皮苷可能与CX 3CL 1蛋白相互作用。结论：柚皮苷通过下调CX 3CL 1和改善线粒体功能来保护HUVEC免受高糖诱导的损伤。
Background/Aims: The induction of endothelial injury by hyperglycemia in diabetes has been widely accepted. Naringin is a bio-flavonoid. Some studies showed that naringin alleviates diabetic complications, but the exact mechanisms by which naringin improves diabetic anomalies are not yet fully understood. The aim of this research was to study the protective effect of naringin on high glucose-induced injury of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured with or without high glucose in the absence or presence of naringin for 5 days. The expression of CX3CL1 was determined by quantitative real-time RT-PCR (qPCR) and western blot. The cellular bioenergetic analysis oxygen consumption rate (OCR) was measured with a Seahorse Bioscience XF analyzer. Results: The production of reactive oxygen species (ROS), the expression of CX3CL1 and the level of AKT phosphorylation were increased in HUVECs cultured with high glucose compared with controls. However, naringin rescued these increases in ROS production, CX3CL1 expression and AKT phosphorylation. Nitric oxide (NO) production and OCR were lower in the high glucose group, and naringin restored the changes induced by high glucose. Molecular docking results suggested that Naringin might interact with the CX3CL1 protein. Conclusion: Naringin protects HUVECs from high-glucose-induced damage through its antioxidant properties by downregulating CX3CL1 and by improving mitochondrial function.