Relationships between rabbit semen characteristics and fertilising ability after insemination.
Relationships between rabbit semen characteristics and fertilising ability after insemination.
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家兔精液特性与授精后受精能力的关系。
DOI：
10.1017/s1751731115002372
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发表时间：
2016
期刊：
影响因子：
3.6
通讯作者：
J. Brun
中科院分区：
文献类型：
--
作者：
M. Theau;E. Ailloud;A. Sánchez;G. Saleil;J. Brun
本研究旨在分析家兔精液特征与授精后精液受精能力之间的关系，一般认为受精能力较弱。我们的假设是，使用高精液稀释度（1:19）、非发情刺激母液和同精授精将更容易预测精液受精能力。对 128 个 INRA1001 雄鹿的 275 次精液进行了评估，并将其分为五个连续批次。总共进行了 1970 次授精。连续精液变量被细分为大小相似的三类，以解释精液特征和受精能力之间的非线性关系。根据显微镜观察下是否存在波，质量运动分为两类。性欲、凝胶的存在与否、体积、前进精子的百分比、曲线速度、鞭毛的跳动频率以及精子运动的直线度和线性度不影响生育力、繁殖力或生产力。经证实，通过在显微镜下目视观察估计的质量运动显着影响生育力以及活动精子和快速精子的百分比，以及通过计算机辅助精液分析系统估计的侧向头部位移的幅度。在较小程度上，运动细胞和快速细胞的百分比显着影响繁殖力。因此，质量运动和运动细胞的百分比显着影响兔母鹿的生产力（当精液至少显示出波浪运动的开始，或当运动细胞的百分比> 84％时，+1活产/AI）。有趣的是，当快速细胞的百分比从 64% 变化到 79% 时，观察到兔子的体重增加了 1.5 只，而生产力显着下降到超过快速细胞的 83%，反映了非线性关系。
This study aimed to analyse the relationship between rabbit semen characteristics and semen fertilising ability after insemination, which is generally found to be weak. Our hypothesis was that using high semen dilutions (1 : 19), non-oestrus-stimulated does, and homospermic inseminations would make it easier to predict semen fertilising ability. Semen characteristics were evaluated on 275 ejaculates of 128 INRA1001 bucks, distributed into five successive batches. A total of 1970 inseminations were performed. The continuous semen variables were subdivided into three classes of similar size to account for any non-linear relationship between semen characteristics and fertilising ability. Mass motility was divided into two classes according to the presence or absence of waves under microscope observation. Libido, the presence or absence of gel, volume, percentage of progressive sperms, curvilinear velocity, beat frequency of the flagellum, and straightness and linearity of sperm movement did not affect fertility, prolificacy or productivity. It was confirmed that mass motility, estimated by visual observation under the microscope, significantly influenced fertility as well as the percentage of motile and of rapid sperms, and the amplitude of lateral head displacement, estimated by a computer-assisted semen analysis system. To a lesser extent, the percentage of motile cells and of rapid cells significantly influenced prolificacy. Consequently, mass motility and the percentage of motile cells significantly influenced rabbit doe productivity (+1 live births/AI when the semen showed at least a beginning of wave movement, or when the percentage of motile cells was >84%). Interestingly, a gain of 1.5 rabbits was observed when the percentage of rapid cells changed from 64% to 79%, whereas productivity significantly dropped beyond 83% of rapid cells, reflecting a non-linear relationship.