Elevated expression of annexin II (lipocortin II, p36) in a multidrug resistant small cell lung cancer cell line.
Elevated expression of annexin II (lipocortin II, p36) in a multidrug resistant small cell lung cancer cell line.
复制标题
在多药抗性小细胞肺癌细胞系中，膜联蛋白II（Lipocortin II，p36）的表达升高。
DOI：
10.1038/bjc.1992.103
复制
发表时间：
1992-04
影响因子：
8.8
通讯作者：
Deeley, R G
中科院分区：
文献类型：
--
作者：
Cole, S P;Pinkoski, M J;Bhardwaj, G;Deeley, R G
阿霉素选择的多药耐药小细胞肺癌细胞系H69AR对长春花生物碱和鬼臼毒素具有交叉耐药，但不过度表达p -糖蛋白，p -糖蛋白是一种170 kDa的质膜外排泵，通常与这种耐药有关。针对H69AR细胞系产生单克隆抗体，其中一种单克隆抗体MAb 3.186可以识别一个36kda磷酸化蛋白上的肽表位，该蛋白与膜相关，但不呈现在H69AR细胞的外表面（Mirski & Cole, 1991）。本研究采用体外翻译和分子克隆技术测定3.186抗原对应mRNA的相对水平。此外，用125I-MAb 3.186筛选H69AR cDNA文库，获得了一个含有约1.4 kb插入片段的cDNA克隆。该克隆DNA片段在H69AR细胞中杂交成一个约1.6 kb的mRNA物种，该物种在H69AR细胞中增加了5至6倍。部分DNA测序和限制性内切酶图谱显示克隆的DNA与p36相同，p36是Ca2+和磷脂结合蛋白的膜联蛋白/脂皮质蛋白家族的成员。
The doxorubicin-selected multidrug resistant small cell lung cancer cell line, H69AR, is cross-resistant to the Vinca alkaloids and epipodophyllotoxins, but does not overexpress P-glycoprotein, a 170 kDa plasma membrane efflux pump usually associated with this type of resistance. Monoclonal antibodies were raised against the H69AR cell line and one of these, MAb 3.186, recognises a peptide epitope on a 36 kDa phosphorylated protein that is membrane associated, but not presented on the external surface of H69AR cells (Mirski & Cole, 1991). In the present study, in vitro translation and molecular cloning techniques were used to determine the relative levels of mRNA corresponding to the 3.186 antigen. In addition, a cDNA clone containing an insert of approximately 1.4 kb was obtained by screening an H69AR cDNA library with 125I-MAb 3.186. Fragments of this cloned DNA hybridised to a single mRNA species of approximately 1.6 kb that was 5 to 6-fold elevated in H69AR cells. Partial DNA sequencing and restriction endonuclease mapping revealed identity of the cloned DNA with p36, a member of the annexin/lipocortin family of Ca2+ and phospholipid binding proteins.