Choline and Ethanolamine Plasmalogens Prevent Lead-Induced Cytotoxicity and Lipid Oxidation in HepG2 Cells
Choline and Ethanolamine Plasmalogens Prevent Lead-Induced Cytotoxicity and Lipid Oxidation in HepG2 Cells
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DOI：
10.1021/acs.jafc.9b02485
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发表时间：
2019-07-10
影响因子：
6.1
通讯作者：
Hui, Shu-Ping
中科院分区：
文献类型：
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作者：
Wu, Yue;Chen, Zhen;Hui, Shu-Ping
来自膳食磷脂的缩醛磷脂被认为是对抗氧化相关疾病的潜在保护剂，而铅（Pb）是世界范围内的环境污染物，并且已知会诱导氧化应激。然而，保护和抗脂质氧化作用的个别plasmalogen物种对铅损伤很少受到关注。在这项研究中，在人肝癌细胞中评价了6种缩醛磷脂（以胆碱或乙醇胺为头基，p16：0/18：1、p16：0/ 18：2或p16：0/20：5为侧链）。缩醛磷脂物质显示出细胞活力的显著恢复以及活性氧的消除，并抑制磷脂酰胆碱氢过氧化物（从63.6 +/- 1.8%至80.3 +/- 2.9%）和磷脂酰乙醇胺氢过氧化物（从25.7 +/- 9.3%至76.1 +/- 3.7%）的蓄积。此外，缩醛血浆显着上调一系列通过Nrf-2依赖性途径调节的抗氧化酶的基因表达水平。本研究表明胆碱和乙醇胺缩醛磷脂可以防止铅诱导的HepG 2细胞毒性和脂质氧化。
Plasmalogens derived from dietary phospholipids are considered to be potential protectors against oxidation related disorders, while lead (Pb) is an environmental contaminant worldwide and is known to induce oxidative stress. However, the protective and antilipid oxidative effects of individual plasmalogen species against Pb damage have received little attention. In this study, six plasmalogen species (with either choline or ethanolamine as the headgroup and p16:0/18:1, p16:0/ 18:2, or p16:0/20:5 as the side chains) were evaluated in human hepatoma cells. Plasmalogen species showed a remarkable recovery in cell viability as well as elimination of reactive oxygen species and suppressed the accumulation of phosphatidylcholine hydroperoxides (from 63.6 +/- 1.8% to 80.3 +/- 2.9%) and phosphatidylethanolamine hydroperoxides (from 25.7 +/- 9.3% to 76.1 +/- 3.7%). Moreover, plasmalogens significantly upregulated the gene expression levels of a series of antioxidant enzymes that are regulated via the Nrf-2-dependent pathway. This study suggested that choline and ethanolamine plasmalogens could prevent Pb-induced cytotoxicity and lipid oxidation in HepG2 cells.