Interfacing microbial styrene production with a biocompatible cyclopropanation reaction.
Interfacing microbial styrene production with a biocompatible cyclopropanation reaction.
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DOI：
10.1002/anie.201502185
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发表时间：
2015-06-08
影响因子：
16.6
通讯作者：
Balskus, Emily P.
中科院分区：
文献类型：
--
作者：
Wallace, Stephen;Balskus, Emily P.
Introducing new reactivity into living organisms is a major challenge in synthetic biology. Despite an increasing interest in both developing aqueous-compatible small molecule catalysts and engineering enzymes to perform new chemistry in vitro, the integration of non-native reactivity into metabolic pathways for small molecule production has been underexplored. Herein we report a biocompatible iron(III) phthalocyanine catalyst capable of efficient olefin cyclopropanation in the presence of a living microorganism. By interfacing this catalyst with E. coli engineered to produce styrene, we synthesize non-natural phenyl cyclopropanes directly from D-glucose in single-vessel fermentations. This process represents the first combination of non-biological carbene-transfer reactivity with cellular metabolism for small molecule production.
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