Distribution of growth hormone-like cells in the pituitary of adult sea lampreys, Petromyzon marinus
Distribution of growth hormone-like cells in the pituitary of adult sea lampreys, Petromyzon marinus
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DOI：
10.2108/zsj.19.1055
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发表时间：
2002-09-01
影响因子：
0.9
通讯作者：
Nozaki, M
中科院分区：
文献类型：
--
作者：
Ominato, K;Nozaki, M
生长激素 (GH)、催乳素 (PRL) 和生长激素 (SL) 是垂体激素家族的成员，据信它们是从共同祖先基因通过复制和随后的分化进化而来的。由于这些激素在硬骨鱼和软骨鱼中都存在，因此它们的祖先形式应该存在于无颌中。因此，虽然没有令人信服的证据表明七鳃鳗垂体分泌 GH 或 PRL，但使用哺乳动物和/或鱼类来源的 GH、PRL 和 SL 抗体，在成年七鳃鳗 (Petromyzon marinus) 的垂体中检查了 GH 和/或 PRL 样免疫反应性。我们最初尝试使用普通免疫组织化学程序未能检测到七鳃鳗垂体中的任何阳性反应。对切片进行水合高压灭菌预处理后，抗鲑鱼 GH、抗鲑鱼 PRL 和抗蓝鲨 GH 在分布于近端远侧部背半部的大多数细胞中出现阳性反应。这些结果表明，与这些抗体发生免疫反应的物质在某种程度上与 GH/PRL 有关，但由于交叉反应性低，需要通过切片的水合高压灭菌预处理来增强免疫反应性以揭示这一点。七鳃鳗和硬骨鱼垂体内的地形分布相似，表明七鳃鳗 GH/PRL 样细胞是七鳃鳗的 GH 细胞。
Growth hormone (GH), prolactin (PRL) and somatolactin (SL) are members of a pituitary hormone family that are believed to have evolved from a common ancestral gene by duplication and subsequent divergence. Since these hormones are found both in bony fish and cartilaginous fish, their ancestral form(s) should be present in the Agnatha. Thus, although there is no convincing evidence that the lamprey pituitary secretes GH or PRL, GH- and/or PRL-like immunoreactivity was examined in the pituitary of adult sea lampreys (Petromyzon marinus), using antibodies to GHs, PRLs and SL of mammalian and/or fish origins. Our initial attempt with ordinary immunohistochemical procedures failed to detect any positive reactions in the lamprey pituitary. Following the hydrated autoclave pretreatment of the sections, anti-salmon GH, anti-salmon PRL and anti-blue shark GH gave positive reactions in most cells distributed in the dorsal half of the proximal pars distalis. These results suggest that the material immunoreactive to those antibodies is related, to some extent, to GH/PRL, but enhancement of immunoreactivity to reveal this by the hydrated autoclave pretreatment of sections is needed due to low cross reactivity. The similarity of the topographic distributions within the pituitary between lampreys and teleosts suggests that lamprey GH/PRL-like cells are GH cells of the lamprey.