An immunohistochemical analysis of cyclin D1, p53. and p21waf1/cip1 proteins in tumors originating from the follicular epithelium of the thyroid gland
An immunohistochemical analysis of cyclin D1, p53. and p21waf1/cip1 proteins in tumors originating from the follicular epithelium of the thyroid gland
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DOI：
10.1078/0344-0338-00037
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Machinami, R
中科院分区：
文献类型：
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作者：
Goto, A;Sakamoto, A;Machinami, R
本研究旨在阐明与甲状腺肿瘤进展密切相关的因素。应用免疫组织化学技术检测179例来源于滤泡上皮的甲状腺肿瘤组织中细胞周期蛋白D1、p53和p21(waf1/cip1)的免疫反应。细胞周期蛋白D_1在高分化甲状腺癌中表达较多(39/122例)，而在滤泡性腺瘤中较少表达(1/33例)，差异有统计学意义(P<0.05)。低分化癌(7/19例)和未分化癌(4/5例)的阳性率明显高于高分化癌(14/122例)(P<0.05)。高分化甲状腺癌中p21(waf1/cip1)阳性率(43/122)明显高于滤泡性腺瘤(4/33)(p<；0.05)。在这些蛋白之间的关系方面，低分化癌(5/7例)中细胞周期蛋白D1和P53的共同阳性率明显高于高分化癌(7/39例)(p<；0.05)。低分化癌(6/7例)中，大多数细胞周期蛋白D1阳性的病例未见p21(waf1/cip1)的表达。3例P53和p21(waf1/cip1)共同阳性，提示细胞周期蛋白D_1与甲状腺肿瘤的发生有关。此外，P53可能与低分化癌和高分化癌的发生发展密切相关。
This study aimed at clarifying the factors closely related to the tumor progression of thyroid neoplasms. We examined the immunoreactivity of cyclin D1, p53, and p21(waf1/cip1) proteins in 179 thyroid tumors originating from the follicular epithelium using an immunohistochemical technique. Cyclin D1 positivity was frequent in well-differentiated thyroid carcinomas (39/122 cases), but it was rarely seen in follicular adenomas (1/33 cases), (p < 0.05). Positivity for p53 was more frequent in poorly differentiated carcinomas (7/19 cases) and undifferentiated carcinomas (4/5 cases) than in well-differentiated carcinomas (14/122 cases) (p < 0,05, respectively). P21(waf1/cip1) positivity was more frequent in well-differentiated thyroid carcinomas (43/122 cases) than in follicular adenomas (4/33 cases) (p < 0.05). Regarding the relationships of these proteins, co-positivity for cyclin D1 and p53 was observed more often in poorly differentiated carcinomas (5/7 cases) than in well-differentiated carcinomas (7/39 cases) (p < 0.05). Most cases with cyclin D1 positivity did not show p21(waf1/cip1) expression in poorly differentiated carcinomas (6/7 cases). Three cases examined showed co-positivity of p53 and p21(waf1/cip1).Our results suggest that cyclin D1 is invoved in thyroid oncogenesis. Moreover, p53 might be closely related to the development of poorly differentiated carcinomas and undifferentiated carcinomas originating from well-differentiated carcinomas.