Isolation and characterization of pathogens attacking Pomacea canaliculata
Isolation and characterization of pathogens attacking Pomacea canaliculata
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DOI：
10.1023/b:wibi.0000007312.97058.48
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发表时间：
2003-12-01
影响因子：
4.1
通讯作者：
Bhumiratana, A
中科院分区：
文献类型：
--
作者：
Chobchuenchom, W;Bhumiratana, A
从泰国不同农业区的土壤样品中分离出7种能杀灭福寿螺的微生物。使用显微镜检查和生化试验对这些微生物进行鉴定。5株菌株被鉴定为铜绿假单胞菌，并命名为铜绿假单胞菌19.1、21.2.1、B1.1、P1和P2。另外2株经鉴定为荧光假单胞菌（Pseudomonasfluorescens），分别命名为荧光假单胞菌13.1和Ct 1。未经高压灭菌处理和高压灭菌处理的细胞悬液对福寿螺的LC_（50）水平为3.56 × 10 ~（4）~ 1.35 × 10 ~（6）c。F. u./ ml和3.09 × 10（4）至1.23 × 10（6）c. F. u./ ml，分别。
Seven microorganisms capable of killing Pomacea canaliculata were isolated from soil samples obtained from various agricultural areas of Thailand. The identification of these microorganisms was performed using microscopic examination and biochemical tests. Five strains were identified as Pseudomonas aeruginosa and were designated P. aeruginosa 19.1, 21.2.1, B1.1, P1 and P2. The other two strains were identified as Pseudomonas fluorescens and were designated P. fluorescens 13.1 and Ct1.Pathogenicity studies of these microorganisms to P. canaliculata (Lamarck) were performed and characterized by LC50 levels. The LC50 levels of non-autoclave-treated and autoclave-treated cell suspensions to P. canaliculata were found to be 3.56 x 10(4) - 1.35 x 10(6) c. f. u./ ml and 3.09 x 10(4) to 1.23 x 10(6) c. f. u./ ml, respectively.