Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence of Giardia ardeae, G. muris, G. duodenalis and Hexamita sp.
Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence of Giardia ardeae, G. muris, G. duodenalis and Hexamita sp.
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双单胞菌的独特系统发育位置基于贾第虫、G. muris、G. duodenalis 和 Hexamita sp 的完整小亚基核糖体 RNA 序列。
DOI：
10.1096/fasebj.7.1.8422968
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Meyer,EA
中科院分区：
文献类型：
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作者：
vanKeulen,H;Gutell,RR;Gates,MA;Campbell,SR;Erlandsen,SL;Jarroll,EL;Kulda,J;Meyer,EA
测定了两种肠道寄生虫贾第鞭毛虫：G. ardeae和G.鞭毛虫的完整小亚单位rRNA （SSU - rRNA）编码区序列。这些序列与贾第鞭毛虫属的第三个成员G. duodenalis（通常称为G.十二指肠鞭毛虫）已发表的SSU - rDNA序列进行了比较。intestinalisorG。蓝藻属)和其他分类群的各种代表性生物。在分析的三个贾第序列中，来自mg的SSU - rRNA。与g的1435和1453个碱基相比，muris最小（1432个碱基）。ardeaeandG。G + C含量最低（58.9%）。hexamitassu - rRNA是这一组中最大的，包含1550个碱基。由于SSU - rRNA的大小是原核生物而不是典型的真核生物，因此构建了SSU - rRNA的二级结构。这些结构显示了一些典型的真核特征序列。基于二级结构约束的序列比对构建了这4个类群的系统发育树。结果表明，在所代表的四种外交昆虫中，贾第虫形成了一个独特的群体。另一种病原六形虫和微孢子虫异形虫与贾第鞭毛虫不同。-van Keulen， H., Gutell， R. R., Gates， M. A., Campbell， S. R., Erlandsen， S. L., Jarroll， E. L., Kulda， J., Meyer， E. A.基于贾第鞭毛虫，G. muris， G. duodenalis和hexamita sp.的完整小亚基核糖体RNA序列的独特系统发育定位；1993.
Complete small‐subunit rRNA (SSU‐rRNA) coding region sequences were determined for two species of the intestinal parasiteGiardia: G. ardeaeandG. muris, both belonging to the order Diplomonadida, and a free‐living member of this order,Hexamita sp.These sequences were compared to published SSU‐rDNA sequences from a third member of the genusGiardia, G. duodenalis(often calledG. intestinalisorG. lamblia) and various representative organisms from other taxa. Of the threeGiardiasequences analyzed, the SSU‐rRNA fromG. murisis the smallest (1432 bases as compared to 1435 and 1453 forG. ardeaeandG. duodenalis, respectively) and has the lowest G + C content (58.9%). TheHexamitaSSU‐rRNA is the largest in this group, containing 1550 bases. Because the sizes of the SSU‐rRNA are prokaryotic rather than typically eukaryotic, the secondary structures of the SSU‐rRNAs were constructed. These structures show a number of typically eukaryotic signature sequences. Sequence alignments based on constraints imposed by secondary structure were used for construction of a phylogenetic tree for these four taxa. The results show that of the four diplomonads represented, theGiardiaspecies form a distinct group. The other diplomonadHexamitaand the microsporidiumVairimorpha necatrixappear to be distinct fromGiardia.—van Keulen, H., Gutell, R. R., Gates, M. A., Campbell, S. R., Erlandsen, S. L., Jarroll, E. L., Kulda, J., Meyer, E. A. Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence ofGiardia ardeae, G. muris, G. duodenalis, andHexamita sp.FASEB J.7: 223‐231; 1993.