Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination.
Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination.
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DOI：
10.4161/rna.7.3.11912
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发表时间：
2010-05
期刊：
影响因子：
4.1
通讯作者：
Werner F
中科院分区：
文献类型：
--
作者：
Grohmann D;Werner F
来自真核生物、古细菌和细菌这三个生命领域的进化相关的多亚单位RNA聚合酶具有共同的结构和功能特性。我们最近发现，两个RNAP亚基F/E(RPB4/7)-在真核细胞和古生菌之间保守，但没有细菌同源物-与新生的RNA链相互作用，从而深刻地调节RNAP活性。总体而言，F/E增加了转录的加工性，但它也以一种依赖于序列的方式刺激转录终止。除了RNA结合，这两个明显相反的过程可能涉及RNAP钳的变构机制。Spt4/5是唯一已知的在生命的所有三个结构域都保守的RNAP相关转录因子，它刺激类似于RNAP亚基F/E的伸长，Spt4/5通过与RNAP钳相互作用，以一种独立于非模板DNA链的方式增强加工性。虽然Spt4/5的分子机制在进化中是普遍保守的，但在细菌和古真核生物谱系分裂后，F/E类复合体的附加功能已经出现。有趣的是，理论上，噬菌体编码的抗终止子蛋白可以在细菌RNAP中发挥类似的功能。
Evolutionary related multisubunit RNA polymerases from all three domains of life, Eukarya, Archaea and Bacteria, have common structural and functional properties. We have recently shown that two RNAP subunits, F/E (RPB4/7)—which are conserved between eukaryotes and Archaea but have no bacterial homologues—interact with the nascent RNA chain and thereby profoundly modulate RNAP activity. Overall F/E increases transcription processivity, but it also stimulates transcription termination in a sequence-dependent manner. In addition to RNA-binding, these two apparently opposed processes are likely to involve an allosteric mechanism of the RNAP clamp. Spt4/5 is the only known RNAP-associated transcription factor that is conserved in all three domains of life, and it stimulates elongation similar to RNAP subunits F/E. Spt4/5 enhances processivity in a fashion that is independent of the nontemplate DNA strand, by interacting with the RNAP clamp. Whereas the molecular mechanism of Spt4/5 is universally conserved in evolution, the added functionality of F/E-like complexes has emerged after the split of the bacterial and archaeo-eukaryotic lineages. Interestingly, bacteriophage-encoded antiterminator proteins could, in theory, fulfil an analogous function in the bacterial RNAP.
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影响因子：
4.1
作者：
Grohmann, Dina;Hirtreiter, Angela;Werner, Finn
通讯作者：
Werner, Finn
影响因子：
5.3
作者：
KOLODZIEJ, PA;WOYCHIK, N;YOUNG, RA
通讯作者：
YOUNG, RA
影响因子：
64.5
作者：
Artsimovitch, I;Patlan, V;Vassylyev, DG
通讯作者：
Vassylyev, DG
影响因子：
16
作者：
Kettenberger, H;Armache, KJ;Cramer, P
通讯作者：
Cramer, P
影响因子：
48
作者：
Muschielok, Adam;Andrecka, Joanna;Michaelis, Jens
通讯作者：
Michaelis, Jens