QUANTITATIVE ANALYSIS OF SPERMATOGENESIS OF RAT - A REVISED MODEL FOR RENEWAL OF SPERMATOGONIA
QUANTITATIVE ANALYSIS OF SPERMATOGENESIS OF RAT - A REVISED MODEL FOR RENEWAL OF SPERMATOGONIA
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DOI：
10.1002/aja.1001110202
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发表时间：
1962-01-01
影响因子：
--
通讯作者：
CLERMONT, Y
中科院分区：
文献类型：
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作者：
CLERMONT, Y
通过分析生精上皮细胞周期各阶段的生殖细胞（A型、In型和B型精原细胞、精母细胞）计数和研究静息和分裂A型干细胞-精原细胞的图谱，研究了成年大鼠精原细胞的更新模式。约10.6%的第二代A型精原细胞在精原细胞五次分裂的第二次分裂前期发生退化。相应地校正了In和B精原细胞以及精母细胞（A型精原细胞有丝分裂产生的元素）的定量数据，并获得了新的校正细胞计数。A型干细胞的图谱显示，它们分组，并且在这些组中它们是配对的。利用校正的细胞计数并考虑干细胞的成对排列，提出了以下“干细胞更新”模型。一对A型干细胞在周期的第IX阶段开始分裂，此后四个子A型细胞在第XII和XIV-I阶段相继分裂，它们的数量以几何级数增加。在第三次分裂后，两个精原细胞从同一个A型细胞中产生，形成一对新的休眠的A型干细胞，随后为精原细胞群的更新提供条件。其它精原细胞变成中间型细胞，其在第IV阶段又分裂产生B型精原细胞，其在第VI阶段又分裂产生初级精母细胞。
The mode of renewal of spermatogonia was investigated in adult rats by analysing counts of germ cells (type A, In and B spermatogonia, spermatocytes) at various stages of the cycle of the seminiferous epithelium and by investigating maps of resting and dividing type A stem-spermatogonia. Some 10.6% of the second generation type A spermatogonia degenerate during prophase of the second of the series of five spermatogonial divisions. The quantitative data on In and B spermatogonia, and on spermatocytes, elements which arise from type A spermatogonial mitoses, were corrected accordingly, and new corrected cell counts were obtained. Maps of type A stem cells revealed that they divided in groups and within these groups they were paired. Utilizing the corrected cell counts and considering the pair arrangement of the stem cells, the following "stem cell renewal" model was proposed. A pair of type A stem cells start dividing at stage IX of the cycle, thereafter the four daughter type A cells divide successively at stages XII and XIV-I, their number increasing in geometrical progression. Following this third division two spermatogonia arising from the same type A cell, form a pair of new dormant type A stem cells which later provide for the renewal of the spermatogonial population. The other spermatogonia become intermediate type cells which in turn divide at stage IV to give type B spermatogonia which in turn divide at stage VI to yield primary spermatocytes.