Evidence for common horizontal transmission of Wolbachia among butterflies and moths.
Evidence for common horizontal transmission of Wolbachia among butterflies and moths.
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DOI：
10.1186/s12862-016-0660-x
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发表时间：
2016-05-27
影响因子：
3.4
通讯作者：
Kawahara AY
中科院分区：
文献类型：
--
作者：
Ahmed MZ;Breinholt JW;Kawahara AY
沃尔巴克氏体是地球上分布最广泛的细菌之一。先前对沃尔巴克氏体与宿主相互作用的研究表明，该细菌通常通过卵细胞质从母体垂直转移到后代。尽管据报道沃尔巴克氏体从一个物种到另一个物种的水平传播在节肢动物中很常见，但可用的直接生态证据有限。在这项研究中，我们使用多位点序列分型（MLST）菌株数据集检查沃尔巴克氏体的水平传播，并使用沃尔巴克氏体和鳞翅目基因组来寻找鳞翅目（最多样化的世界性昆虫目之一）横向基因转移（LGT）的证据。我们构建了节肢动物相关 MLST Wolbachia 菌株的系统发育，并校准了与鳞翅目物种相关的 Wolbachia 菌株的年龄。我们的结果揭示了沃尔巴克氏菌菌株的种间、属间、科间和序数间水平传播，但没有明显的地理模式。我们在鳞翅目内的 31 个物种之间以及鳞翅目与其他节肢动物宿主之间发现了至少 7 例可能的水平传播病例。分歧时间分析显示，Wolbachia 最近（22.6–4.7 mya，95% HPD）引入鳞翅目。对九种鳞翅目基因组（家蚕、Danaus plexippus、Heliconius melpomene、Manduca sexta、Melitaea cinxia、Papilio glaucus、P. polytes、P. xuthus 和 Plutella xylostella）的分析得出了一种可能的 Wolbachia LGT 实例。我们的研究结果提供了蝴蝶和飞蛾中相同和多种沃尔巴克氏体菌株高发病率的证据，将鳞翅目添加到越来越多的沃尔巴克氏体常见水平传播的证据中。这项研究展示了这种非凡且有影响力的微生物的有趣动态。本文的在线版本 (doi:10.1186/s12862-016-0660-x) 包含补充材料，可供授权用户使用。
Wolbachia is one of the most widespread bacteria on Earth. Previous research on Wolbachia-host interactions indicates that the bacterium is typically transferred vertically, from mother to offspring, through the egg cytoplasm. Although horizontal transmission of Wolbachia from one species to another is reported to be common in arthropods, limited direct ecological evidence is available. In this study, we examine horizontal transmission of Wolbachia using a multilocus sequence typing (MLST) strains dataset and used Wolbachia and Lepidoptera genomes to search for evidence for lateral gene transfer (LGT) in Lepidoptera, one of the most diverse cosmopolitan insect orders. We constructed a phylogeny of arthropod-associated MLST Wolbachia strains and calibrated the age of Wolbachia strains associated with lepidopteran species. Our results reveal inter-specific, inter-generic, inter-familial, and inter-ordinal horizontal transmission of Wolbachia strains, without discernible geographic patterns. We found at least seven probable cases of horizontal transmission among 31 species within Lepidoptera and between Lepidoptera and other arthropod hosts. The divergence time analysis revealed that Wolbachia is recently (22.6–4.7 mya, 95 % HPD) introduced in Lepidoptera. Analysis of nine Lepidoptera genomes (Bombyx mori, Danaus plexippus, Heliconius melpomene, Manduca sexta, Melitaea cinxia, Papilio glaucus, P. polytes, P. xuthus and Plutella xylostella) yielded one possible instance of Wolbachia LGT. Our results provide evidence of high incidence of identical and multiple strains of Wolbachia among butterflies and moths, adding Lepidoptera to the growing body of evidence for common horizontal transmission of Wolbachia. This study demonstrates interesting dynamics of this remarkable and influential microorganism. The online version of this article (doi:10.1186/s12862-016-0660-x) contains supplementary material, which is available to authorized users.