Metabolism of alcohol at high concentrations: role and biochemical nature of the hepatic microsomal ethanol oxidizing system.
Metabolism of alcohol at high concentrations: role and biochemical nature of the hepatic microsomal ethanol oxidizing system.
复制标题
高浓度酒精的代谢：肝微粒体乙醇氧化系统的作用和生化性质。
DOI：
10.1007/978-1-4899-5181-6_18
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发表时间：
1977
影响因子：
--
通讯作者：
C. Lieber
中科院分区：
文献类型：
--
作者：
R. Teschke;S. Matsuzaki;K. Ohnishi;Y. Hasumura;C. Lieber
在中等和较高的酒精浓度下，乙醇代谢通过乙醇脱氢酶（ADH）和微粒体乙醇氧化系统（MEOS）进行，而过氧化氢酶不起重要作用。长期消耗乙醇后，MEOS 活性和乙醇代谢率都会增强；尽管吡唑抑制 ADH 和氧化钠抑制过氧化氢酶，但后者仍持续存在，表明 MEOS 参与了适应性增加。 MEOS 表现出与其他微粒体药物代谢酶相似的特征，并且可以通过 ADH 和过氧化氢酶活性来区分和分离。在合成磷脂存在的情况下，使用部分纯化的细胞色素 P-450 和 NADPH-细胞色素 c 还原酶实现了 MEOS 活性的重建。
At intermediate and higher alcohol concentrations, ethanol metabolism proceeds via alcohol dehydrogenase (ADH) and the microsomal ethanol oxidizing system (MEOS), whereas catalase plays no significant role. Following prolonged ethanol consumption, an enhancement of both MEOS activity as well as the rates of ethanol metabolism occurs; the latter persisted despite inhibition of ADH by pyrazole and catalase by sodium axide, suggesting the involvement of MEOS in the adaptive increase. MEOS exhibits characteristics similar to those of other microsomal drug metabolizing enzymes and can be differentiated and isolated from both ADH and catalase activities. Reconstitution of MEOS activity was achieved with partially purified cytochrome P-450 and NADPH-cytochrome c reductase in the presence of synthetic phospholipid.