Role of Syzygium cumini seed extract in the chemoprevention of in vivo genomic damage and oxidative stress
Role of Syzygium cumini seed extract in the chemoprevention of in vivo genomic damage and oxidative stress
复制标题
DOI：
10.1016/j.jep.2010.12.014
复制
发表时间：
2011-03-24
影响因子：
5.4
通讯作者：
Premkumar, Kumpati
中科院分区：
文献类型：
--
作者：
Arun, Renganathan;Prakash, M. Velayutham Dass;Premkumar, Kumpati
乙醇药理学相关性：Syzygium cumini，Skeels（Jamun）的种子在印度被广泛用于治疗糖尿病和其他疾病。研究的目的：这项工作的目的是评估Jamun种子提取物（JSE）作为化学保护剂对抗体内氧化应激和基因组损伤的作用。材料和方法：进行实验以评价JSE对羟基自由基诱导的pBR 322 DNA损伤的体外保护作用，以及在暴露于遗传毒性致癌物尿烷（URE）和7，12-二甲基苯并蒽（DMBA）之前口服JSE 5天的小鼠体内基因组损伤和氧化应激。JSE的水提物和醇提物对羟基自由基诱导的pBR 322 DNA链断裂有明显的保护作用。在体内实验与水JSE显示出显着的保护作用，对染色体损伤引起的遗传毒性致癌物的URE和DMBA。生化检测显示，JSE能显著抑制肝脏脂质过氧化反应，提高GSH水平和GST、SOD、CAT活性。结论：JSE可能作为抗氧化应激和基因组损伤的化学预防剂发挥重要作用。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Ethanopharmacological relevance: The seeds of Syzygium cumini, Skeels (Jamun) are extensively used in India for treatment of diabetes and other ailments.Aim of the study: The aim of this work was to assess the role of Jamun seed extract (JSE) as a chemoprotective agent against in vivo oxidative stress and genomic damage.Materials and methods: Experiments were carried out to evaluate in vitro protective effects of JSE against hydroxyl radical induced damage in pBR322 DNA, and in vivo genomic damage and oxidative stress in mice which received JSE orally for 5 days before exposure to genotoxic carcinogens urethane (URE) and 7,12-dimethyl benz(a)anthracene (DMBA).Results: Aqueous and ethanolic extracts of JSE showed significant protective effects against hydroxyl radical induced strand breaks in pBR322 DNA. The in vivo experiments with aqueous JSE showed significant protective effects against chromosomal damage induced by the genotoxic carcinogens URE and DMBA. Biochemical assays registered significant inhibition of hepatic lipid peroxidation and increase in GSH level and activity of GST, SOD and CAT.Conclusion: Our findings suggest that JSE can possibly play an important role as a chemopreventive agent against oxidative stress and genomic damage. (C) 2010 Elsevier Ireland Ltd. All rights reserved.