A new mechanical stimulator for cultured bone cells using piezoelectric actuator.
A new mechanical stimulator for cultured bone cells using piezoelectric actuator.
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DOI：
10.1016/s0021-9290(98)00183-3
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发表时间：
1999-04
影响因子：
2.4
通讯作者：
S. Tanaka
中科院分区：
文献类型：
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作者：
S. Tanaka
在这项研究中，一种新的机械刺激器，利用压电驱动器，使培养的骨细胞产生更多的生理大小、频率分量和波形的机械应变。该刺激器为骨细胞提供了三维胶原凝胶块培养的机械应变，应变大小为200-40,000μ，频率为DC-100 Hz，充分覆盖了骨上的生理应变。此外，该刺激器不仅可以产生正弦波和矩形波等常见的应变波形，还可以在个人计算机上合成任意应变波形。特别是，应变频率和波形的可控性比以前的刺激器更有进步。因此，该设备可以促进关于骨细胞对机械刺激的反应的新发现。
In this study, a new mechanical stimulator using the piezoelectric actuator was developed to give cultured bone cells mechanical strains with more physiologic magnitude, frequency components, and waveform. This stimulator provides bone cells in a three-dimensional collagen gel block culture mechanical strains with magnitude of 200–40,000μ strain and frequency of DC –100Hz, which sufficiently covers physiological strains on bone. Furthermore, the stimulator can generate not only common strain waveforms like sine and rectangular waves, but also arbitrary strain waveforms synthesized on a personal computer. In particular, the controllability of strain frequency and waveform is an advance over that of previous stimulators. Thus, this device can facilitate new findings regarding bone cell responses to mechanical stimuli.