Establishment of continuous cultures of T-cell acute lymphoblastic leukemia cells at diagnosis.
Establishment of continuous cultures of T-cell acute lymphoblastic leukemia cells at diagnosis.
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DOI：
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发表时间：
1990
期刊：
影响因子：
11.2
通讯作者：
R. Gjerset;A. Yu;M. Haas
中科院分区：
文献类型：
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作者：
R. Gjerset;A. Yu;M. Haas
我们已经设计了方法，以便于建立连续培养的T细胞母细胞从患者的急性淋巴细胞白血病的T细胞型诊断。培养的细胞在表面标志物、核型和T细胞受体基因重排方面与诊断时的患者细胞非常相似。培养的T细胞急性淋巴细胞白血病（诊断）细胞（a）是具有卷曲核的淋巴细胞;（B）具有24-48小时的倍增时间;（c）依赖于白细胞介素2生长;（d）是逆转录酶阴性的;（e）在甲基纤维素中不形成集落;和（f）就T细胞受体β链重排而言是克隆的。三个T细胞急性淋巴细胞白血病培养物具有正常的二倍体核型，并且一个具有在诊断时也存在的6 q缺失。
We have devised methods facilitating the establishment of continuous cultures of T-cell blasts from patients with acute lymphoblastic leukemia of T-cell type at diagnosis. The cultured cells closely resemble those of the patients at the time of diagnosis with respect to surface markers, karyotype, and T-cell receptor gene rearrangements. Cultured T-cell acute lymphoblastic leukemia (diagnosis) cells (a) are lymphocytes with a convoluted nucleus; (b) have doubling times of 24-48 h; (c) are dependent for growth on interleukin 2; (d) are reverse transcriptase negative; (e) do not form colonies in methyl cellulose; and (f) are clonal with respect to T-cell receptor beta chain rearrangements. Three T-cell acute lymphoblastic leukemia cultures had a normal diploid karyotype, and one had a 6q- deletion which was also present at the time of diagnosis.