Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)
Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)
复制标题
与尖镰孢抗性相关的 RAPD 和 AFLP 标记分析。
DOI：
10.1007/s10681-012-0665-5
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发表时间：
2012
期刊：
影响因子：
1.9
通讯作者：
N. Hayashida
中科院分区：
文献类型：
--
作者：
Daisuke Aruga;N. Tsuchiya;H. Matsumura;E. Matsumoto;N. Hayashida
莴苣根腐病是由尖孢镰刀菌引起的。莴苣根瘤菌（FOL）是莴苣生产中的关键问题。已经鉴定了FOL-resistant莴苣遗传资源并将其用于育种计划以产生FOL-resistant栽培品种。然而，抗性基因的遗传特性尚未被深入研究，因此，目前没有DNA标记可用于这些基因。在这项研究中，我们分析了RRD 2（根腐病2号小种的抗性）基因座，它赋予了对FOL小种2的抗性。利用两个脆头莴苣品种：VP 1013（抗病）和爱国者（感病）的抗性位点进行了分析。F2代各抗性表型的分离模式为单一主效基因座。为了确定抗性位点的位置，利用扩增片段长度多态性和随机扩增多态性DNA（RAPD）标记构建了连锁图。数量性状位点分析揭示了主效抗性位点的位置。RAPD标记WF 25 -42的LOD值较高，与不同品种和品系的表型有较好的对应关系。我们成功地开发了一个序列特征的扩增区域标记从WF 25 -42。
Root rot of lettuce, which is caused byFusarium oxysporumf. sp.lactucae(FOL), is a critical problem in the production of lettuce. FOL-resistant lettuce genetic resources have been identified and used in breeding programs to produce FOL-resistant cultivars. However, the genetic characteristics of resistance genes have not been studied in depth and, therefore, no DNA markers are presently available for these genes. In this study, we analyzed theRRD2(resistance for root rot disease race 2) locus, which confers resistance to FOL race 2. Resistance loci were analyzed using two cultivars of crisphead lettuce: VP1013 (resistant) and Patriot (susceptible). The segregation patterns of resistant phenotypes in F2indicated a single major locus. To define the positions of resistance loci, a linkage map was constructed using amplified fragment length polymorphism and random amplified polymorphic DNA (RAPD) markers. Quantitative trait loci analysis revealed the position of the major resistance locus. A high LOD score was observed for RAPD-marker WF25-42, and this marker showed good correspondence to the phenotype in different cultivars and lines. We successfully developed a sequence characterized amplified region marker from WF25-42.