Dependence of DNA Persistence Length on Ionic Strength and Ion Type
Dependence of DNA Persistence Length on Ionic Strength and Ion Type
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DOI：
10.1103/physrevlett.122.028102
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发表时间：
2019-01-18
影响因子：
8.6
通讯作者：
Tardin, Catherine
中科院分区：
文献类型：
--
作者：
Guilbaud, Sebastien;Salome, Laurence;Tardin, Catherine
尽管双链DNA的持久长度L-P在细胞生物学和纳米技术中起着关键作用，但其对离子强度I的依赖性缺乏一致的描述。使用高通量单分子技术和统计物理建模，我们在单价（Li+，Na+，K+）和二价（Mg 2+，Ca 2+）金属和烷基铵离子存在下测量L-P，在0.5 mM的大范围内
Even though the persistence length L-P of double-stranded DNA plays a pivotal role in cell biology and nanotechnologies, its dependence on ionic strength I lacks a consensual description. Using a high-throughput single-molecule technique and statistical physics modeling, we measure L-P in the presence of monovalent (Li+, Na+, K+) and divalent (Mg2+, Ca2+) metallic and alkyl ammonium ions, over a large range 0.5 mM