Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound
Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound
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DOI：
10.1016/j.cplett.2015.08.054
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发表时间：
2015-10-01
影响因子：
2.8
通讯作者：
Yoshikawa, Kenichi
中科院分区：
文献类型：
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作者：
Ma, Yue;Ogawa, Naoki;Yoshikawa, Kenichi
抗坏血酸对DNA双链断裂的保护作用是通过荧光显微镜观察巨DNA（T4 DNA; 166 kbp）的单分子来评价的。样品暴露于三种不同形式的辐射：可见光、伽马射线和超声波。对于可见光照射，1 mM AA将损伤降低至约100 nm。百分之三十相同浓度的AA可降低γ射线对Ca.百分之七十然而，AA对超声波引起的损伤几乎没有保护作用。这种显着的差异进行了讨论的物理化学机制的双链断裂取决于辐射源。(C)2015作者由Elsevier B. V.发布，这是CC BY许可下的开放获取文章。
The protective effect of ascorbic acid against double-strand breaks in DNA was evaluated by singlemolecule observation of giant DNA (T4 DNA; 166 kbp) through fluorescence microscopy. Samples were exposed to three different forms of radiation: visible light, gamma-ray and ultrasound. With regard to irradiation with visible light, 1 mM AA reduced the damage down to ca. 30%. Same concentration of AA decreased the damage done by gamma-ray to ca. 70%. However, AA had almost no protective effect against the damage caused by ultrasound. This significant difference is discussed in relation to the physico-chemical mechanism of double-strand breaks depending on the radiation source. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.