Quantal release of serotonin
Quantal release of serotonin
复制标题
DOI：
10.1016/s0896-6273(00)00097-0
复制
发表时间：
2000-10-01
期刊：
影响因子：
16.2
通讯作者：
Jahn, R
中科院分区：
文献类型：
--
作者：
Bruns, D;Riedel, D;Jahn, R
我们研究了小突触囊泡（ssv）和大密包囊泡（ldcv）量子变异的起源。作为一个模型，我们使用水蛭的5 -羟色胺能Retzius神经元，允许结合电流和形态分析量子递质释放。我们发现，SSV释放的递质量与囊泡的体积成正比，表明5 -羟色胺以恒定的囊泡内浓度储存，并在胞吐过程中完全排出。ldcv的发射机放电表现出比其大小分布预期的更高程度的变异性，并且ldcv的大量释放比ssv的释放慢。平均而言，从ssv和ldcv中释放的递质量的差异与细胞器的大小差异成正比，这表明ssv和ldcv中的递质储存浓度相似。
We have studied the origin of quantal Variability for small synaptic Vesicles (SSVs) and large dense-cored vesicles (LDCVs). As a model, we used serotonergic Retzius neurons of leech that allow for combined amperometrical and morphological analyses of quantal transmitter release. We find that the transmitter amount released by a SSV varies proportionally to the volume of the vesicle, suggesting that serotonin is stored at a constant intravesicular concentration and is completely discharged during exocytosis. Transmitter discharge from LDCVs shows a higher degree of variability than is expected from their size distribution, and bulk release from LDCVs is slower than release from SSVs. On average, differences in the transmitter amount released from SSVs and LDCVs are proportional to the size differences of the organelles, suggesting that transmitter is stored at similar concentrations in SSVs and LDCVs.