ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan
ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan
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DOI：
10.1016/s0014-5793(00)01854-8
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发表时间：
2000-08-04
期刊：
影响因子：
3.5
通讯作者：
Matsushima, K
中科院分区：
文献类型：
--
作者：
Kuno, K;Okada, Y;Matsushima, K
具有血小板反应蛋白I型基序的去整合素样金属蛋白酶-1（ADAMTS-1）是细胞外基质锚定的金属蛋白酶。在本研究中，我们证明了ADAMTS-1能够切割主要的软骨蛋白聚糖-聚集蛋白聚糖，切割产物的N-末端测序分析显示ADAMTS-1切割聚集蛋白聚糖硫酸软骨素附着结构域中的Glu（1871）-Leu（1872）键。此外，缺失分析表明ADAMTS-1的C-末端间隔区是降解聚集蛋白聚糖所必需的，这些结果表明ADAMTS-1可能参与了聚集蛋白聚糖在体内的周转。(C)2000欧洲生物化学学会联合会，由Elsevier Science B. V.出版，版权所有。
A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan, N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan, In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan, These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.