Photoperiodic control of FT-like gene ClFT initiates flowering in Chrysanthemum lavandulifolium
Photoperiodic control of FT-like gene ClFT initiates flowering in Chrysanthemum lavandulifolium
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DOI：
10.1016/j.plaphy.2013.11.004
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发表时间：
2014-01-01
影响因子：
6.5
通讯作者：
Dai, Silan
中科院分区：
文献类型：
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作者：
Fu, Jianxin;Wang, Linlin;Dai, Silan
FLOW LOCUS T（FT）基因在调节从营养期到生殖期的过渡中起着至关重要的作用。本研究从菊花中克隆了一个FT同源基因（ClFT）。序列分析表明，ClFT基因启动子含有光响应、脱落酸、干旱诱导响应和昼夜节律钟等多个元件。观察了ClFT在不同发育阶段不同组织器官中的表达模式及其对不同光周期的响应。ClFT在所有测试的器官/组织中表达，在短日照（SD）条件下，在具有可见花芽的植物的叶中观察到最高表达水平。接下来，我们研究了ClFT在不同光周期处理期间的节律性表达，发现ClFT的水平随着连续黑暗时间的增加而增加。ClFT在连续暗期为12 h时积累，与光期长短无关。野生型拟南芥（Col-0）中ClFT基因的异位表达导致提早开花，在转基因拟南芥中观察到内源LFY和SOC 1的高表达水平。以上结果表明，ClFT基因在短日照诱导下促进棉花开花的作用具有进化保守性。该基因可作为菊花开花时间遗传操作的重要靶基因。(C)2013年Elsevier Masson SAS。All rights reserved.
The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative phase to the reproductive phase. In this study, we isolated an FT homologous gene (denoted as ClFT) from Chrysanthemum lavandulifolium. The sequencing analysis indicated that the promoter of the ClFT gene contains many elements, such as light response, abscisic acid, drought-inducibility response and CIRCADIAN clock elements. The expression patterns of ClFT in different tissues/organs at different developmental stages and its responses to different photoperiods were observed. ClFT is expressed in all tested organs/tissues, with the highest expression level being observed in the leaves of plants with visible floral buds under the short day (SD) condition. Next, we studied the rhythmic expression of ClFT during different photoperiod treatments and found that the level of ClFT increases with additional hours of continuous dark. ClFT accumulates when the continuous dark period is 12 h, regardless of the duration of light period. The ectopic expression of the ClFT gene in wild type Arabidopsis (Col-0) results in early flowering, with high expression levels of endogenous LFY and SOC1 being observed in transgenic Arabidopsis. All results indicated that the ClFT gene plays an evolutionarily conserved role in promoting flowering in inductive short days in C. lavandulifolium and that this gene could serve as a vital target for the genetic manipulation of flowering time in chrysanthemums. (C) 2013 Elsevier Masson SAS. All rights reserved.