Seven Amino Acid Types Suffice to Create the Core Fold of RNA Polymerase
Seven Amino Acid Types Suffice to Create the Core Fold of RNA Polymerase
复制标题
七种氨基酸类型足以创建 RNA 聚合酶的核心折叠
DOI：
10.1021/jacs.1c05367
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发表时间：
2021
影响因子：
15
通讯作者：
and Shunsuke Tagami*
中科院分区：
文献类型：
--
作者：
Sota Yagi;Aditya K. Padhi;Jelena Vucinic;Sophie Barbe;Thomas Schiex;Reiko Nakagawa;David Simoncini*;Kam Y. J. Zhang*;and Shunsuke Tagami*
The extant complex proteins must have evolved from ancient short and simple ancestors. The double-ψ β-barrel (DPBB) is one of the oldest protein folds and conserved in various fundamental enzymes, such as the core domain of RNA polymerase. Here, by reverse engineering a modern DPBB domain, we reconstructed its plausible evolutionary pathway started by “interlacing homodimerization” of a half-size peptide, followed by gene duplication and fusion. Furthermore, by simplifying the amino acid repertoire of the peptide, we successfully created the DPBB fold with only seven amino acid types (Ala, Asp, Glu, Gly, Lys, Arg, and Val), which can be coded by only GNN and ARR (R = A or G) codons in the modern translation system. Thus, the DPBB fold could have been materialized by the early translation system and genetic code.
