Segmentation of the rabies virus genome
Segmentation of the rabies virus genome
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DOI：
10.1016/j.virusres.2018.05.017
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发表时间：
2018-07-02
期刊：
影响因子：
5
通讯作者：
Itou, Takuya
中科院分区：
文献类型：
--
作者：
Hidaka, Yuya;Lim, Chang-Kweng;Itou, Takuya
我们建立了一个系统，用于回收的分段重组狂犬病毒，病毒基因组RNA分为两部分：节段1编码的核蛋白，磷蛋白，基质蛋白和糖蛋白基因，和节段2编码的大RNA依赖的RNA聚合酶基因。分段重组狂犬病病毒的形态为子弹状，长度约为130 nm，比200 nm长的非分段重组狂犬病病毒短。将分段重组狂犬病病毒保持至少18代。在传代过程中，分段重组狂犬病病毒的病毒增殖率低于非分段重组狂犬病病毒，并且分段重组狂犬病病毒感染细胞的上清液中片段1和片段2的病毒基因组RNA的相对量不同。这些结果表明，分段重组狂犬病病毒包装片段1或片段2到每个病毒颗粒。因此，与包装节段1或节段2的节段重组狂犬病病毒颗粒的共感染可能是生产子代病毒所必需的。
We established a system for the recovery of a segmented recombinant rabies virus, the virus genome RNA of which was divided into two parts: segment 1 encoding the nucleoprotein, phosphoprotein, matrix protein, and glycoprotein genes, and segment 2 encoding the large RNA-dependent RNA polymerase gene. The morphology of the segmented recombinant rabies virus was bullet-like in shape with a length of approximately 130 nm, which is shorter than the 200-nm long non-segmented recombinant rabies virus. The segmented recombinant rabies virus was maintained for at least 18 passages. The virus multiplication rate of the segmented recombinant rabies virus was lower than that of the non-segmented recombinant rabies virus during the passages, and the relative amounts of virus genome RNAs for segment 1 and segment 2 differed in the supernatant of the segmented recombinant rabies virus infected cells. These results suggest that the segmented recombinant rabies virus packages either segment 1 or segment 2 into each virus particle. Thus, co-infection with segmented recombinant rabies virus particles packaging segment 1 or segment 2 may be necessary for the production of progeny virus.