Convergence of quantum dot barcodes with microfluidics and signal processing for multiplexed high-throughput infectious disease diagnostics
Convergence of quantum dot barcodes with microfluidics and signal processing for multiplexed high-throughput infectious disease diagnostics
复制标题
DOI：
10.1021/nl071415m
复制
发表时间：
2007-09-01
期刊：
影响因子：
10.8
通讯作者：
Chan, Warren C. W.
中科院分区：
文献类型：
--
作者：
Klostranec, Jesse M.;Xiang, Qing;Chan, Warren C. W.
通过纳米技术和微技术(量子点和微流体)的融合，我们创造了一个能够对人类血清样本中的传染病病原体进行多路高通量分析的诊断系统。作为概念验证，我们展示了检测全球最流行的血液传播传染病(即乙肝、丙型肝炎和艾滋病毒)的血清生物标记物的能力，样本量低(L 100亩)，快速(1小时)，灵敏度是目前FDA批准的方法的50倍。我们进一步展示了同时检测血清中多个生物标记物的精确度，且交叉反应最小。该设备可以进一步开发成便携式手持式医疗保健诊断系统，这将代表着在检测、监测、治疗和防止传染病在发达国家和发展中国家传播方面的重大进步。
Through the convergence of nano- and microtechnologies (quantum dots and microfluidics), we have created a diagnostic system capable of multiplexed, high-throughput analysis of infectious agents in human serum samples. We demonstrate, as a proof-of-concept, the ability to detect serum biomarkers of the most globally prevalent blood-borne infectious diseases (i.e., hepatitis B, hepatitis C, and HIV) with low sample volume (< 100 mu L), rapidity (< 1 h), and 50 times greater sensitivity than that of currently available FDA-approved methods. We further show precision for detecting multiple biomarkers simultaneously in serum with minimal cross-reactivity. This device could be further developed into a portable handheld point-of-care diagnostic system, which would represent a major advance in detecting, monitoring, treating, and preventing infectious disease spread in the developed and developing worlds.