Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopy.
Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopy.
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DOI：
10.1038/nchem.961
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发表时间：
2011-03
期刊：
影响因子：
21.8
通讯作者：
Tao, Nongjian
中科院分区：
文献类型：
--
作者：
Wang, Wei;Foley, Kyle;Shan, Xiaonan;Wang, Shaopeng;Eaton, Seron;Nagaraj, Vinay J.;Wiktor, Peter;Patel, Urmez;Tao, Nongjian
报道了一种基于表面等离子体共振的电化学阻抗显微镜。新的EIM可以解决局部阻抗与亚微米的空间分辨率，并监测动态的各种过程，如细胞凋亡和电穿孔的单个细胞与毫秒的时间分辨率。高空间和时间分辨率的图像不仅可以研究单个细胞，还可以在没有标记的情况下解析亚细胞结构和过程。用电流设置实现的检测灵敏度为~2 pS，考虑到单个离子通道的电导在5-400 pS的范围内，这是优异的。我们还描述了一个模型，模拟EIM图像的细胞的基础上，当地的介电常数和电导率。
We report an electrochemical impedance microscope (EIM) based on surface plasmon resonance. The new EIM can resolve local impedance with sub-micron spatial resolution, and monitor dynamics of various processes, such as apoptosis and electroporation of individual cells with millisecond time resolution. The high spatial and temporal resolution images make it possible to not only study individual cells, but also resolve the sub-cellular structures and processes without labels. The detection sensitivity achieved with the current setup is ~2 pS, which is excellent considering the conductance of a single ion channel is in the range of 5–400 pS. We describe also a model that simulates the EIM images of cells based on local dielectric constant and conductivity.
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影响因子：
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