Two-dimensional Self-assembly of a Designed Amphiphilic Peptide at Air/Water Interface
Two-dimensional Self-assembly of a Designed Amphiphilic Peptide at Air/Water Interface
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DOI：
10.1295/polymj.pj2008154
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发表时间：
2008-12
期刊：
影响因子：
2.8
通讯作者：
Masayoshi Tanaka;K. Ogura;Souhei Abiko;N. Koshikawa;T. Kinoshita
中科院分区：
文献类型：
--
作者：
Masayoshi Tanaka;K. Ogura;Souhei Abiko;N. Koshikawa;T. Kinoshita
设计了具有疏水性和亲水性氨基酸残基交替序列的肽（QAQL）4（CH 3CO-（Gln-Ala-Gln-Leu）4-NH 2），以在空气/水界面形成β-折叠单层。通过设计以不带电荷的氨基酸为亲水性残基的肽，在较宽的pH范围内均能形成稳定的单分子膜。采用LB法制备的肽单分子膜呈纳米纤维状排列。（QAQL）4-PEG也是通过在空气/水界面处的自组装来构建纳米结构的有吸引力的构建块。附着在肽段上的PEG表现出从扁平状态到刷状状态的构象转变，这取决于水亚相上的单层膜的压缩程度。构象转变的同时，诱导的纳米结构的形态变化，从盘状结构的纤维阵列。
A peptide having alternate sequence of hydrophobic and hydrophilic amino acid residues, (QAQL)4 (CH3CO-(Gln-Ala-Gln-Leu)4-NH2), was designed to form a β-sheet monolayer at the air/water interface. According to the designing of the peptide having charge free amino acid as hydrophilic residues, the peptide showed stable monolayer at wide range pH. The peptide monolayer prepared by Langmuir-Blodgett (LB) method showed an arranged nano-fibrous object. (QAQL)4-PEG was also an attractive building block for construction of nano-architecture by self-assembly at the air/water interface. PEG attached on the peptide segment showed a conformational transition from a flattened state to brush one, dependent on the compressing degree of the monolayer on water subphase. The conformational transition was simultaneously induced a morphological change of the nano-architecture from disk-like structure to fibrous array.