CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro
CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro
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DOI：
10.1007/s00011-020-01349-7
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发表时间：
2020-07-01
影响因子：
6.7
通讯作者：
Costantini, Todd W.
中科院分区：
文献类型：
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作者：
Chan, Theresa W.;Langness, Simone;Costantini, Todd W.
目的与设计CHRFAM 7A是一个独特的人类基因，编码α 7烟碱乙酰胆碱受体的显性负性抑制剂。我们最近发现CHRFAM 7A在人白细胞中表达，增加细胞-细胞粘附，并调节与白细胞迁移相关的基因的表达。材料人THP-1、RAW 264.7和HEK 293细胞。方法采用细胞迁移、细胞增殖、软琼脂集落形成等方法比较CHRFAM 7A基因转染细胞和载体细胞的生物学活性。结果我们显示，CHRFAM 7A基因递送到THP-1人单核细胞系中减少了细胞迁移，减少了对单核细胞趋化蛋白的趋化性，并减少了软琼脂中的集落形成。结论总而言之，研究结果表明CHRFAM 7A调节单核细胞/巨噬细胞的生物活性，使其在体外迁移并进行非贴壁依赖性生长。
Objective and design CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the alpha 7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration. Material Human THP-1, RAW264.7 and HEK293 cells. Methods Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. Results We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. Conclusion Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.