Severe periodontitis may influence cementum and dental pulp through inflammation, oxidative stress, and apoptosis
Severe periodontitis may influence cementum and dental pulp through inflammation, oxidative stress, and apoptosis
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严重的牙周炎可能通过炎症、氧化应激和细胞凋亡影响牙骨质和牙髓
DOI：
10.1002/jper.18-0604
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发表时间：
2019-11-01
影响因子：
4.3
通讯作者：
Wang, Songlin
中科院分区：
文献类型：
--
作者：
Li, Xiangchun;Hu, Liang;Wang, Songlin
背景慢性牙周炎与牙髓/牙骨质改变的关系很少报道。本研究旨在观察重度慢性牙周炎患者牙骨质的显微结构变化和牙髓的组织病理学特征。方法收集80颗重度慢性牙周炎磨牙和50颗正常拔除的第三磨牙作为对照。扫描电镜观察牙骨质的显微结构，苏木精-伊红染色。通过免疫组织化学/蛋白质印迹法检测白细胞介素（IL）-17和IL-1 β水平。同时检测血清活性氧（ROS）和超氧化物歧化酶1（SOD 1）水平. Caspase 3表达和末端脱氧核苷酸转移酶缺口末端标记（TUNEL）法检测细胞凋亡，LC 3B和P62检测细胞自噬水平。结果牙骨质表面凹凸不平，牙髓炎症，氧化应激。IL-17和IL-1 β水平在牙周炎的牙髓中增加。牙髓组织中ROS和细胞凋亡水平均高于正常牙髓组织，而SOD 1水平低于正常牙髓组织.有趣的是，自噬标记物LC 3B和P62在从牙周炎牙齿获得的牙髓中上调。结论重度慢性牙周炎对牙骨质和牙髓的微观结构均有影响。从牙周炎的牙齿收集的牙髓发炎，在氧化应激下，并提出了相对于正常牙髓的细胞凋亡和自噬水平增加。
Background The relationship between chronic periodontitis and pulpal/cemental changes is seldom reported. This study aimed to report on the microstructural changes of cementum and histopathological features of the dental pulp in teeth with severe chronic periodontitis. Methods Eighty molar teeth with severe chronic periodontitis and 50 extracted third molars (as normal controls) were collected. The microstructure of cementum was evaluated by scanning electron microscopy, and the pulp was stained with hematoxylin and eosin. Interleukin (IL)-17 and IL-1 beta levels were examined by immunohistochemistry/western blotting. Reactive oxygen species (ROS) and superoxide dismutase 1 (SOD 1) levels were also checked. Caspase 3 expression and terminal-deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used as apoptotic indices, and LC3B and P62 were detected to demonstrate the level of autophagy. Results The surface of cementum showed irregularities; the dental pulp was inflamed and under oxidative stress. IL-17 and IL-1 beta levels were increased in the pulp of teeth with periodontitis. ROS and apoptosis levels were higher than in normal dental pulp, while SOD 1 level was reduced. Intriguingly, autophagy markers LC3B and P62 were upregulated in the pulp obtained from teeth with periodontitis. Conclusions Severe chronic periodontitis influenced the microstructure of both cementum and dental pulp. The dental pulp collected from teeth with periodontitis was inflamed, under oxidative stress, and presented increased levels of apoptosis and autophagy relative to normal dental pulp.