Liquid chromatographic assay of dityrosine in human cerebrospinal fluid
Liquid chromatographic assay of dityrosine in human cerebrospinal fluid
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DOI：
10.1016/s0378-4347(97)00248-x
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发表时间：
1997-08-29
期刊：
影响因子：
--
通讯作者：
Verma, A
中科院分区：
文献类型：
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作者：
Abdelrahim, M;Morris, E;Verma, A
建立了一种液-液萃取-离子对反相高效液相色谱荧光检测分离测定人脑脊液中二酪氨酸的方法。含有1-庚磺酸的移动的流动相在甲醇中从0线性增加到100%，超过30分钟，使得二酪氨酸与其他荧光化合物分离，激发波长为285 nm，发射波长为410 nm。可以利用少至0.15ml的CSF样品，在柱上的检测限为60 pg二酪氨酸。该方法便于使用CSF二酪氨酸作为中枢神经系统中自由基介导的蛋白质损伤的量度。(C)1997年Elsevier Science B.V.
A micro-scale method for separation and measurement of dityrosine in human cerebrospinal fluid (CSF) is described utilizing liquid-liquid extraction and ion-paired, reversed-phase high-performance liquid chromatography with fluorimetric detection. A mobile phase containing 1-heptanesulfonic acid linearly increased in methanol from 0 to 100% over 30 min allows the resolution of dityrosine from other fluorescent compounds with excitation at 285 nm and emission at 410 nm. As little as 0.15 mi CSF sample can be utilized with a detection limit of 60 pg dityrosine on the column. This method facilitates the use of CSF dityrosine as a measure of free radical mediated protein damage in the central nervous system. (C) 1997 Elsevier Science B.V.