Maternal Protein Restriction and Post-Weaning High-Fat Feeding Alter Plasma Amino Acid Profiles and Hepatic Gene Expression in Mice Offspring.
Maternal Protein Restriction and Post-Weaning High-Fat Feeding Alter Plasma Amino Acid Profiles and Hepatic Gene Expression in Mice Offspring.
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DOI：
10.3390/foods11050753
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发表时间：
2022-03-04
期刊：
影响因子：
--
通讯作者：
Kato H
中科院分区：
文献类型：
--
作者：
Miyoshi M;Saito K;Jia H;Kato H
母亲在怀孕期间营养不良与儿童的表观遗传变化密切相关，并影响儿童一生中肥胖的发展。在这里，我们研究胎儿低蛋白暴露和断奶后高脂肪消费对血浆氨基酸谱和肝脏基因表达的影响。在妊娠期间，母亲C57 BL/6 J小鼠喂食20%（CN）或9%（LP）酪蛋白饮食。出生后，这两组的雄性后代从6到32周喂食高脂肪饮食（HF）。在32周时，两组之间的最终体重保持不变，但LP-HF组显示出明显更高的白色脂肪重量和血浆瘦素水平。LP-HF组的异亮氨酸、亮氨酸、组氨酸、苯丙氨酸、丝氨酸和酪氨酸浓度显著增加。然而，肝脏中的脂质含量未观察到差异。根据肝脏基因表达分析，LP-HF组显著上调参与染色质修饰/组织途径的基因。因此，母体低蛋白和断奶后的高脂肪环境导致了严重的肥胖状态和后代肝染色质修饰相关基因表达的变化。这些发现为在生命早期阶段预防与生活方式相关的疾病提供了新的见解。
Maternal undernutrition during pregnancy is closely associated with epigenetic changes in the child, and it affects the development of obesity throughout the child’s life. Here, we investigate the effect of fetal low protein exposure and post-weaning high-fat consumption on plasma amino acid profiles and hepatic gene expression. Mother C57BL/6J mice were fed a 20% (CN) or 9% (LP) casein diet during pregnancy. After birth, the male offspring of both these groups were fed a high-fat diet (HF) from 6 to 32 weeks. At 32 weeks, the final body weight between the two groups remained unchanged, but the LP-HF group showed markedly higher white fat weight and plasma leptin levels. The LP-HF group exhibited a significant increase in the concentrations of isoleucine, leucine, histidine, phenylalanine, serine, and tyrosine. However, no differences were observed in the lipid content in the liver. According to the hepatic gene expression analysis, the LP-HF group significantly upregulated genes involved in the chromatin modification/organization pathways. Thus, maternal low protein and a post-weaning high-fat environment contributed to severe obesity states and changes in gene expression related to hepatic chromatin modification in offspring. These findings provide novel insights for the prevention of lifestyle-related diseases at the early life stage.
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影响因子：
3.9
作者：
O'Shaughnessy A;Hendrich B
通讯作者：
Hendrich B
影响因子：
9.3
作者：
Ruiz-Canela M;Toledo E;Clish CB;Hruby A;Liang L;Salas-Salvadó J;Razquin C;Corella D;Estruch R;Ros E;Fitó M;Gómez-Gracia E;Arós F;Fiol M;Lapetra J;Serra-Majem L;Martínez-González MA;Hu FB
通讯作者：
Hu FB
DOI：
10.1016/j.bbrc.2017.02.101
发表时间：
2017-04-29
影响因子：
3.1
作者：
Zhang, Ling;Han, Junqing
通讯作者：
Han, Junqing
影响因子：
--
作者：
van Straten, Esther M. E.;Bloks, Vincent W.;Plosch, Torsten
通讯作者：
Plosch, Torsten
影响因子：
4
作者：
Vo, ThinXuan;Revesz, Andrew;Hardy, Daniel B.
通讯作者：
Hardy, Daniel B.