A stress-associated protein, AtSAP13, from Arabidopsis thaliana provides tolerance to multiple abiotic stresses
A stress-associated protein, AtSAP13, from Arabidopsis thaliana provides tolerance to multiple abiotic stresses
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DOI：
10.1111/pce.13103
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发表时间：
2018-05-01
影响因子：
7.3
通讯作者：
Dhankher, Om Parkash
中科院分区：
文献类型：
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作者：
Dixit, Anirudha;Tomar, Parul;Dhankher, Om Parkash
植物中的胁迫相关蛋白（Stress-Associated Protein，SAP）家族成员具有多种非生物胁迫耐受性，但它们在提供多种非生物胁迫耐受性中的作用方式尚不清楚。拟南芥中有14个SAP基因，含有A20、AN 1和Cys 2-His 2锌指结构域。AtSAP 13是SAP家族的成员，携带两个AN 1锌指结构域和一个额外的Cys 2-His 2结构域。AtSAP 13转录表现出上调响应镉，阿坝和盐胁迫。AtSAP 13过表达株系表现出较强的耐受有毒金属（砷III，镉，锌），干旱和盐胁迫。此外，转基因株系积累了显着更高的量的锌，但较少的As和Cd积累在地上部和根部。AtSAP 13启动子-GUS融合研究表明，GUS表达主要在维管组织，积水管，和顶端分生组织和区域的根成熟和伸长，以及根毛。在亚细胞水平，AtSAP 13-eGFP融合蛋白定位于细胞核和细胞质中。通过酵母单杂交实验，我们鉴定了几个与AtSAP 13启动子相互作用的AP 2/EREBP家族转录因子。拟南芥AtSAP 13基因受多种非生物胁迫的差异调控，AtSAP 13基因在植物中的表达与植物的生长发育密切相关。AtSAP 13的过表达提供了对有毒金属（砷、镉和锌）、干旱和盐的强耐受性。DNA-蛋白质相互作用酵母单杂交试验确定了几个非生物胁迫相关的转录因子，如AP 2/EREBP家族成员与AtSAP 13启动子相互作用。AtSAP 13及其同源物将有助于开发气候适应性作物。
Members of Stress-Associated Protein (SAP) family in plants have been shown to impart tolerance to multiple abiotic stresses, however, their mode of action in providing tolerance to multiple abiotic stresses is largely unknown. There are 14 SAP genes in Arabidopsis thaliana containing A20, AN1, and Cys2-His2 zinc finger domains. AtSAP13, a member of the SAP family, carries two AN1 zinc finger domains and an additional Cys2-His2 domain. AtSAP13 transcripts showed upregulation in response to Cd, ABA, and salt stresses. AtSAP13 overexpression lines showed strong tolerance to toxic metals (AsIII, Cd, and Zn), drought, and salt stress. Further, transgenic lines accumulated significantly higher amounts of Zn, but less As and Cd accumulation in shoots and roots. AtSAP13 promoter-GUS fusion studies showed GUS expression predominantly in the vascular tissue, hydathodes, and the apical meristem and region of root maturation and elongation as well as the root hairs. At the subcellular level, the AtSAP13-eGFP fusion protein was found to localize in both nucleus and cytoplasm. Through yeast one-hybrid assay, we identified several AP2/EREBP family transcription factors that interacted with the AtSAP13 promoter. AtSAP13 and its homologues will be highly useful for developing climate resilient crops.AtSAP13 gene from Arabidopsis thaliana is differentially regulated by various abiotic stresses. Overexpression of AtSAP13 provided strong tolerance to toxic metals (arsenic, cadmium, and zinc), drought, and salt. DNA-protein interactions using yeast one-hybrid assay identified several abiotic stress related transcription factors such as AP2/EREBP family members that interacted with the AtSAP13 promoter. AtSAP13 and its homologues will be useful for developing climate resilient crops.