Cell death atlas of the postnatal mouse ventral forebrain and hypothalamus: Effects of age and sex
Cell death atlas of the postnatal mouse ventral forebrain and hypothalamus: Effects of age and sex
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DOI：
10.1002/cne.23298
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发表时间：
2013-08-01
影响因子：
2.5
通讯作者：
Forger, Nancy G.
中科院分区：
文献类型：
--
作者：
Ahern, Todd H.;Krug, Stefanie;Forger, Nancy G.
自然发生的细胞死亡对于哺乳动物神经系统的发育至关重要。尽管几十年来人们一直认识到发育细胞死亡的重要性，但对小鼠大脑各区域的细胞死亡还没有全面的解释。此外，腹侧前脑和下丘脑细胞死亡的几个区域性别差异已被描述，但尚不清楚这种现象有多普遍。我们使用激活的 caspase-3 的免疫组织化学检测来识别出生后 (P) 1 至 P11 的雄性和雌性小鼠大脑中的死亡细胞。测定下丘脑和腹侧前脑 16 个区域的细胞死亡密度、垂死细胞总数和区域体积（下丘脑前部、弓状核、前腹侧室周核、内侧视前核、室旁核、视交叉上核和下丘脑腹内侧核；基底外侧、 中央和内侧杏仁核；终纹床核的侧核和主核；尾壳核；苍白球；侧隔膜；和卡莱哈群岛）。所有区域都显示出年龄对细胞死亡的显着影响。细胞死亡峰值的时间在 P1 到 P7 之间有所不同，并且细胞死亡率的平均速率在不同区域之间变化十倍。检测到细胞死亡和/或区域体积的几个显着的性别差异。这些数据解决了发育文献中的巨大空白，并表明下丘脑和腹侧前脑细胞死亡的发生率和时间存在有趣的区域特异性差异。 J.Comp。内罗尔。 521:2551-2569，2013 年。(c) 2013 年 Wiley 期刊公司。
Naturally occurring cell death is essential to the development of the mammalian nervous system. Although the importance of developmental cell death has been appreciated for decades, there is no comprehensive account of cell death across brain areas in the mouse. Moreover, several regional sex differences in cell death have been described for the ventral forebrain and hypothalamus, but it is not known how widespread the phenomenon is. We used immunohistochemical detection of activated caspase-3 to identify dying cells in the brains of male and female mice from postnatal day (P) 1 to P11. Cell death density, total number of dying cells, and regional volume were determined in 16 regions of the hypothalamus and ventral forebrain (the anterior hypothalamus, arcuate nucleus, anteroventral periventricular nucleus, medial preoptic nucleus, paraventricular nucleus, suprachiasmatic nucleus, and ventromedial nucleus of the hypothalamus; the basolateral, central, and medial amygdala; the lateral and principal nuclei of the bed nuclei of the stria terminalis; the caudate-putamen; the globus pallidus; the lateral septum; and the islands of Calleja). All regions showed a significant effect of age on cell death. The timing of peak cell death varied between P1 to P7, and the average rate of cell death varied tenfold among regions. Several significant sex differences in cell death and/or regional volume were detected. These data address large gaps in the developmental literature and suggest interesting region-specific differences in the prevalence and timing of cell death in the hypothalamus and ventral forebrain. J. Comp. Neurol. 521:2551-2569, 2013. (c) 2013 Wiley Periodicals, Inc.