Chromatin immunoprecipitation of replication factors moving with the replication fork.
Chromatin immunoprecipitation of replication factors moving with the replication fork.
复制标题
随复制叉移动的复制因子的染色质免疫沉淀。
DOI：
10.1007/978-1-60327-815-7_10
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Noguchi,Eishi
中科院分区：
文献类型：
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作者：
Rapp,JordanB;Ansbach,AlisonB;Noguchi,Chiaki;Noguchi,Eishi
染色体的复制涉及多种复制蛋白，包括 DNA 聚合酶、DNA 解旋酶和其他辅助因子。已知许多这些蛋白质定位于复制叉并作为复制体复合物的组成部分与它们一起移动。其他蛋白质不随复制叉移动，但仍然在 DNA 复制中发挥重要作用。因此，为了了解 DNA 复制及其控制的机制，检查每个复制因子的定位非常重要。在这里，我们描述了一种染色质免疫沉淀 (ChIP) 方法，用于在复制叉处定位复制因子。定义复制蛋白的定位应该为理解 DNA 复制过程的调控机制提供重要的见解。
Replication of chromosomes involves a variety of replication proteins including DNA polymerases, DNA helicases, and other accessory factors. Many of these proteins are known to localize at replication forks and travel with them as components of the replisome complex. Other proteins do not move with replication forks but still play an essential role in DNA replication. Therefore, in order to understand the mechanisms of DNA replication and its controls, it is important to examine localization of each replication factor. Here we describe a chromatin immunoprecipitation (ChIP) method to locate a replication factor at the replication fork. Defining the localization of replication proteins should provide important insight into mechanistic understanding of the regulation of the DNA replication process.