Cryptic diversity of Japanese Diaphanosoma (Crustacea: Cladocera) revealed by morphological and molecular assessments
Cryptic diversity of Japanese Diaphanosoma (Crustacea: Cladocera) revealed by morphological and molecular assessments
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DOI：
10.5268/iw-5.3.847
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发表时间：
2015-01
期刊：
影响因子：
3.1
通讯作者：
Csilla Lakatos;J. Urabe;W. Makino
中科院分区：
文献类型：
--
作者：
Csilla Lakatos;J. Urabe;W. Makino
摘要通过形态学观察和线粒体16 S核糖体RNA和细胞色素c氧化酶亚基I基因（mtCOI）的DNA序列分析，我们从日本的多个湖泊中发现了5种不同的Diaphanosoma。此前，D。brachyurum被认为存在于包括日本在内的温带地区;然而，我们没有发现D.日本的短尾草属。我们找到了D。参见amurensis主要分布在高海拔和高纬度的自然生境;参见dubium，杜氏杜父鱼D.参见orientalis和D.参见macrophthalma，它发生在各种栖息地，从大型天然湖泊到低海拔的小型人工池塘。我们还发现了另一个物种，只有一个池塘，拥有mtCOI序列，几乎完全匹配，从北美标本，因此可能是非土著Diaphanosoma。形态鉴定和遗传分析结果的一致性表明，本研究中建立的DNA条形码是有用的，以确定Diaphanosoma物种在日本和邻近地区。
Abstract We found 5 distinct species of Diaphanosoma from a variety of lakes in Japan according to morphological examination and genetic analyses with the DNA sequences of mitochondrial 16S ribosomal RNA and cytochrome c oxidase subunit I genes (mtCOI). Previously, D. brachyurum was thought to occur at temperate regions including Japan; however, we did not find D. brachyurum in Japan. Instead, we found D. cf. amurensis, which inhabited mainly natural habitats of high altitude and latitude, and D. cf. dubium, D. cf. orientalis, and D. cf. macrophthalma, which occurred in a variety of habitats from large natural lakes to small artificial ponds at low altitude. We also found another species from only one pond that possessed mtCOI sequences that matched almost completely with those from North American specimens and thus was probably nonindigenous Diaphanosoma. Concordance between the results of morphological identifications and genetic analyses showed that the DNA barcodes established in this study are useful to identify Diaphanosoma species in Japan and adjacent areas.