Glial cell line-derived neurotrophic factor receptor GFRα1 is expressed in the rat striatum during postnatal development
Glial cell line-derived neurotrophic factor receptor GFRα1 is expressed in the rat striatum during postnatal development
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DOI：
10.1016/j.molbrainres.2004.05.015
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发表时间：
2004-08-23
期刊：
影响因子：
--
通讯作者：
Burke, RE
中科院分区：
文献类型：
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作者：
Cho, JW;Yarygina, O;Burke, RE
黑质（SN）的多巴胺神经元经历一个自然的细胞死亡事件，这是双相的，峰值在出生后第2天和第14天。越来越多的证据表明GDNF作为纹状体靶源性神经营养因子调节第一阶段。目前尚不清楚GDNF受体GFR α 1是否在调节这两个阶段中发挥作用。为了评估GFR α 1的可能作用，我们研究了其在SN和特别是纹状体中的整个出生后发育过程中的表达，其中其表达一直不确定。如前所示，GFR α 1 mRNA在SN中高度表达，在PND 14 -28时水平最高。我们发现，它也表达在纹状体具有类似的时间过程，但与PND 10和PND 14之间的最大表达更离散的时期。这种最大表达的细胞基础是均匀分布在纹状体内的GFR α 1 mRNA阳性中型神经元数量增加。免疫染色显示GFRa 1蛋白阳性神经元具有相似的形态和分布。我们的结论是，GFRalpha 1在纹状体表达最大的后期在出生后的发展。在这个位置，它可以反式影响黑质多巴胺神经元的活力和发育。(C)2004 Elsevier B. V.保留所有权利。
Dopamine neurons of the substantia nigra (SN) undergo a natural cell death event which is biphasic, with peaks at postnatal days (PNDs) 2 and 14. There is growing evidence that GDNF functions as a striatal target-derived neurotrophic factor to regulate the first phase. It has been unknown whether the GDNF receptor, GFRalpha1, may play a role in regulating either phase. To evaluate a possible role for GFRalpha1 we have examined its expression throughout postnatal development in the SN and particularly in the striatum, where its expression has been uncertain. GFRalpha1 mRNA is highly expressed in SN, as previously shown, with highest levels at PND14-28. We find that it is also expressed in striatum with a similar time course, but with a more discrete period of maximal expression between PND10 and PND14. The cellular basis of this maximum of expression is an increased number of GFRalpha1 mRNA-positive medium-sized neurons evenly distributed within the striatum. Immunostaining reveals GFRalpha1 protein-positive neurons with a similar morphology and distribution. We conclude that GFRalpha1 is expressed in striatum maximally late in postnatal development. In this location it may act in trans to influence the viability and development of nigral dopamine neurons. (C) 2004 Elsevier B.V. All rights reserved.