Degradation of Water‐Insoluble Dyes by Microperoxidase‐11, an Effective and Stable Peroxidative Catalyst in Hydrophilic Organic Media
Degradation of Water‐Insoluble Dyes by Microperoxidase‐11, an Effective and Stable Peroxidative Catalyst in Hydrophilic Organic Media
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DOI：
10.1021/bp010124v
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发表时间：
2002
影响因子：
2.9
通讯作者：
H. Wariishi;Mari Kabuto;J. Mikuni;Masafumi Oyadomari;Hiroo Tanaka
中科院分区：
文献类型：
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作者：
H. Wariishi;Mari Kabuto;J. Mikuni;Masafumi Oyadomari;Hiroo Tanaka
微过氧化物酶-11（MP-11）是一种含有血红素的十一肽，来源于马心细胞色素c，用作过氧化催化剂。以2，6-二甲氧基苯酚为还原底物，在一系列不同浓度的有机溶剂中研究了MP-11在亲水性有机介质中的催化特性，结果表明MP-11在水溶性有机溶剂中具有活性，但至少5%的水是催化作用的必需条件。因此，MP-11在疏水性溶剂中无活性。介质中水部分的pH影响反应速率。发现最佳pH为9，其中促进质子从氧化或还原底物释放到介质中。尝试了MP-11在90%甲醇中对水不溶性合成染料的脱色。MP-11对偶氮或蒽醌染料显示出有效的脱色活性。详细研究了溶剂橙子7的降解途径，表明MP-11催化偶氮键的氧化裂解，生成1，2-萘醌和2，4-二甲基苯酚作为关键中间体。
Microperoxidase‐11 (MP‐11), a heme‐containing undecapeptide, derived from horse heart cytochrome c was utilized as a peroxidative catalyst. Catalytic characteristics of MP‐11 in hydrophilic organic media were studied using 2,6‐dimethoxyphenol as a reducing substrate in a series of organic solvents at various concentrations, indicating that MP‐11 was active in water‐miscible organic solvents but at least 5% water was compulsory for the catalytic action. Thus, MP‐11 was not active in hydrophobic solvents. The pH of the water portion in the media affected the reaction rate. The optimal pH was found to be 9, where a release of protons from either an oxidizing or reducing substrate to the media was facilitated. The decolorization of water‐insoluble synthetic dyes by MP‐11 in 90% methanol was attempted. MP‐11 showed effective decolorization activities against either azo or anthraquinone dyes. The degradation pathway for Solvent Orange 7 was investigated in detail, showing that MP‐11 catalyzed the oxidative cleavage of the azo linkage to generate 1,2‐naphthoquinone and 2,4‐dimethylphenol as key intermediates.