In vitro estimation of the rate of hexose phosphorylation, by sequential pulsing with [3H]- and [14C]2-deoxy-D-glucose.
In vitro estimation of the rate of hexose phosphorylation, by sequential pulsing with [3H]- and [14C]2-deoxy-D-glucose.
复制标题
通过连续脉冲[3H]-和[14C]2-脱氧-D-葡萄糖体外估计己糖磷酸化速率。
DOI：
10.1016/0305-0491(85)90122-1
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Crane,PD
中科院分区：
文献类型：
--
作者：
Cornford,EM;Crane,PD
The rate of phosphorylation of 2-deoxy-D-glucose (2dGlc) was determined by incubating Schistosoma mansoni in vitro in [3H] 2-deoxy-D-glucose; 60 sec after exposure to the [3H] dGlc,[14C] dGlc was added to the medium, and metabolic activity was arrested at 2 min by immersion of the tissue in ice-cold silicone oil. Column chromatographic separation of the neutral [3H]-and [14C] dGlc from the [3H]-and [14C] 2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H] dGlc phosphorylated in 2 min, and the proportion of [14C] dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. In male schistosomes derived from mouse infections 4.4+/-0.8% of the dGlc was phosphorylated each minute, and 4.2+/-0.9% in the females. Lower rates of phosphorylation were measured in schistosomes taken from hamsters where males phosphorylated 2.4+/-1.1% of the dGlc each minute, and in females 2.7+/-1.0%. These studies suggest the high rate of hexose utilization by schistosomes compares to the conscious rat brain, where 11% of the dGlc is phosphorylated each minute.
