Neuronal-glial interactions: quantitation of astrocytic influences on development of catecholamine neurons.
Neuronal-glial interactions: quantitation of astrocytic influences on development of catecholamine neurons.
复制标题
神经元-胶质细胞相互作用：定量星形胶质细胞对儿茶酚胺神经元发育的影响。
DOI：
10.1007/bf00965932
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发表时间：
1989
影响因子：
4.4
通讯作者：
Lauder,JM
中科院分区：
文献类型：
--
作者：
Lieth,E;Towle,AC;Lauder,JM
本文研究了星形胶质细胞对胚胎大鼠中脑儿茶酚胺神经元形态和生化分化的影响，并与成纤维细胞的结果进行了比较。神经突起的生长和复杂性进行了测量，使用计算机辅助的形态测量酪氨酸羟化酶免疫反应神经元上的星形胶质细胞或成纤维细胞的预制单层。星形胶质细胞的存在刺激了这些神经元的形态分化，并且这种效应在各种细胞隔室中是明显的，包括细胞索马的大小、神经突和神经炎节段的长度以及这些节段的数量。酪氨酸羟化酶活性测定生化在这些文化中，也被发现刺激存在的星形胶质细胞单层。这些结果的理解胚胎脑发育过程中特定的神经元-胶质细胞的相互作用的影响进行了讨论。
Effects of astroglia on the morphological and biochemical differentiation of catecholamine neurons from embryonic rat mesencephalon were studied in vitro, and compared to results obtained with fibroblasts. Neurite outgrowth and complexity were measured using computer-assisted morphometry on tyrosine hydroxylase immunoreactive neurons growing on preformed monolayers of astrocytes or fibroblasts. The morphological differentiation of these neurons was stimulated by the presence of astrocytes, and this effect was evident in various cellular compartments, including the size of the cell soma, length of neurites and neuritic segments, and the numbers of these segments. Tyrosine hydroxylase activity was measured biochemically in these cultures and was also found to be stimulated by the presence of astroglial monolayers. The implication of these results for the understanding of specific neuron-glial interactions during embryonic brain development is discussed.