CLK-1 protein has DNA binding activity specific to OL region of mitochondrial DNA
CLK-1 protein has DNA binding activity specific to OL region of mitochondrial DNA
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DOI：
10.1016/s0014-5793(02)02568-1
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发表时间：
2002-04-10
期刊：
影响因子：
3.5
通讯作者：
Seluanov, A
中科院分区：
文献类型：
--
作者：
Gorbunova, V;Seluanov, A
秀丽隐杆线虫（cerzorhahditis elegans）的clk-1基因突变可以延长线虫的寿命，减缓多种生理过程。在这里，我们报道秀丽隐杆线虫CLK-1及其小鼠同源物具有特异性的线粒体DNA O-L区域的DNA结合活性。CLK-1的DNA结合活性被ADP抑制，并被延长线虫寿命的突变所改变。我们的研究结果表明，CLK-1除了在泛素生物合成中的酶促功能外，还参与mtDNA复制或转录的调控。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Mutations in the clk-1 gene of Caerzorhahditis elegans extend worm life span and slow down a variety of physiological processes. Here we report that C. elegans CLK-1 as well as its mouse homologue have DNA binding activity that is specific to the O-L region of mitochondrial DNA. DNA binding activity of CLK-1 is inhibited by ADP, and is altered by mutations that extend nematode life span. Our results suggest that, in addition to its enzymatic function in ubiquinone biosynthesis, CLK-1 is involved in the regulation of mtDNA replication or transcription. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.