Visuospatial attentional functioning in mice: interactions between cholinergic manipulations and genotype
Visuospatial attentional functioning in mice: interactions between cholinergic manipulations and genotype
复制标题
DOI：
10.1046/j.1460-9568.1999.00701.x
复制
发表时间：
1999-08-01
影响因子：
3.4
通讯作者：
Wilkinson, LS
中科院分区：
文献类型：
--
作者：
Humby, T;Laird, FM;Wilkinson, LS
小鼠的注意力功能是在类似于五个选择的系列反应时间任务中评估的，在该任务中，要求检测呈现在五个空间位置的短暂视觉刺激。对两个杂交品系：F1、C57BL/6xDBA/2和C57BL/6x129sv进行了鉴定。这两个菌株都获得了高水平的性能，特别是没有因为过早反应而出现问题。在执行阶段，对任务参数的系统操作表明了一种与任务一致的效果模式，这对视觉空间注意的各个方面造成了负担。在不同压力下，基线任务操作的影响存在一定的差异。然而，影响的模式表明，这些很可能是除注意力功能本身以外的因素影响的结果，如行为反应和抑制。在两个品系中都有证据表明东莨菪碱对注意功能具有特异性的中枢调节作用，其中C57BL/6xDBA/2杂交种对药物操纵表现出更高的敏感性。0.02 mg/kg和0.2 mg/kg剂量对鉴别准确率有显著影响。在2 mg/kg剂量下，准确度的大幅下降与对其他测量的重大影响有关，包括遗漏和反应潜伏期，这表明对任务绩效的非特异性影响。这些数据首次表明，操作性方法在评估小鼠的视觉空间注意功能方面是有效的。他们证实了胆碱能机制在跨物种注意过程中的重要性，并提出了胆碱能机制与注意表型表达中的基因型之间的相互作用。
Attentional functioning in mice was assessed in an analogue of the five-choice serial reaction time task in which the requirement was to detect brief visual stimuli presented across five spatial locations. Two hybrid strains of mice were assessed; F1 C57Bl/6xDBA/2 and C57Bl/6x129sv. Both strains acquired the task to high levels of performance with, in particular, no problems due to premature responding. At performance, systematic manipulation of the task parameters indicated a pattern of effects consistent with the task, taxing aspects of visuospatial attention. There were some differential effects of task manipulations at baseline across strain. However, the pattern of effects suggested these were likely to be the result of effects on factors other than attentional functioning per se, such as behavioural reactivity and inhibition. There was evidence in both strains of specific, centrally mediated effects of scopolamine on attentional functioning, with the C57Bl/6xDBA/2 hybrid showing greater sensitivity to the drug manipulation. Specific effects on discriminative accuracy were observed at doses of 0.02 and 0.2 mg/kg scopolamine. At the 2 mg/kg dose, large reductions in accuracy were associated with large effects on other measures, including omissions and response latencies, suggestive of nonspecific effects on task performance. These data indicate, for the first time, the utility of operant methods in assessing visuospatial attentional functioning in mice. They confirm the importance of cholinergic mechanisms in attentional processes across species, and suggest interactions between cholinergic mechanisms and genotype in the expression of attentional phenotypes.