Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n=9) wild relatives
Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n=9) wild relatives
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DOI：
10.1007/s00122-006-0461-5
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发表时间：
2007-02-01
影响因子：
5.4
通讯作者：
King, G. J.
中科院分区：
文献类型：
--
作者：
Allender, C. J.;Allainguillaume, J.;King, G. J.
代表芸苔属“C”（n = 9）基因组的171份材料的叶绿体基因组多样性，包括驯化和野生B。利用6个叶绿体微卫星（SSR）标记，对甘蓝（oleracea）和9个互育近缘野生种的遗传多样性进行了研究。在105份栽培和野生B材料中检测到的多样性不足。与其野生亲戚形成鲜明对比。绝大多数的B。甘蓝属的种质资源共享一个单倍型，而在两个野生种B中检测到多达六个单倍型。维略萨岛和B。cretica Lam.. SSR在单倍型上表现出高度多态性，计算的遗传多样性值（H）为0.23-0.87。在C基因组物种中总共检测到23种不同的单倍型，在B中检测到另外5种单倍型。Rapa L.（A基因组n = 10）和另一个在B中。[医]黑根属（B基因组，n = 8）。B的叶绿体多样性较低。甘蓝并不暗示多重驯化事件。占优势的B。甘蓝单倍型在B中也很常见。因卡纳十号并且在B中以低频存在。villosa，B. macrocarpa Guss，B.岩蕨和B。克瑞提卡叶绿体SSR揭示了丰富的野生芸苔属物种的多样性，这将有助于进一步的进化和植物地理学研究这一重要的作物属。
Diversity in the chloroplast genome of 171 accessions representing the Brassica 'C' (n = 9) genome, including domesticated and wild B. oleracea and nine inter-fertile related wild species, was investigated using six chloroplast SSR (microsatellite) markers. The lack of diversity detected among 105 cultivated and wild accessions of B. oleracea contrasted starkly with that found within its wild relatives. The vast majority of B. oleracea accessions shared a single haplotype, whereas as many as six haplotypes were detected in two wild species, B. villosa Biv. and B. cretica Lam.. The SSRs proved to be highly polymorphic across haplotypes, with calculated genetic diversity values (H) of 0.23-0.87. In total, 23 different haplotypes were detected in C genome species, with an additional five haplotypes detected in B. rapa L. (A genome n = 10) and another in B. nigra L. (B genome, n = 8). The low chloroplast diversity of B. oleracea is not suggestive of multiple domestication events. The predominant B. oleracea haplotype was also common in B. incana Ten. and present in low frequencies in B. villosa, B. macrocarpa Guss, B. rupestris Raf. and B. cretica. The chloroplast SSRs reveal a wealth of diversity within wild Brassica species that will facilitate further evolutionary and phylogeographic studies of this important crop genus.